Isomeric Sugar Recognition Using Molecularly Imprinted Polymer Hydrogels

摘要提案题目：利用分子印迹聚合物水凝胶识别同分异构体糖提案号：cts -0303951项目负责人：Peter kofinas机构：美国马里兰大学。本研究的目的是制备并测试具有分子印迹识别不同材料特性的聚合物水凝胶。分子印迹聚合物（MIP）将被合成并对特定的单糖异构体进行印迹。将开发用于检测和定量每个分子印记的方法。MIP的总糖结合、特异性和糖扩散率将在水和生理介质中进行评估。通过组合合成方法优化糖的特异性识别。一种非共价印迹方法将在水溶液中进行。机制研究将试图阐明分子形状和功能对选择性的作用。就更广泛的影响而言，新的MIPs可能为生物识别和异构体分离技术提供新的途径。本研究开发的MIPs可与色谱技术结合用于生物分离，以分离氨基酸、糖、药物、病毒和其他生物活性化合物。在以前的工作中，已经制定了K-12活动。在这个项目中，将开发一个K-12互动网站。

AbstractProposal Title: Isomeric Sugar Recognition Using Molecularly Imprinted Polymer HydrogelsProposal Number: CTS-0303951Principal Investigator: Peter KofinasInstitution: University of MarylandThe objective of the proposed research is to prepare and test polymer hydrogels that have been molecularly imprinted with recognition properties for different materials. Molecularly imprinted polymers (MIP) will be synthesized and imprinted against specific simple sugar isomers. Methods will be developed for the detection and quantification of each molecular imprint. The MIP's total sugar binding, specificity, and rate of sugar diffusion will be evaluated in aqueous and physiological media. Specific sugar recognition will be optimized through combinatorial synthesis methods. A non-covalent approach to imprinting will be carried out in aqueous solution. Mechanistic studies will attempt to clarify the roles of molecular shape and functionality on selectivity. In terms of the broader impacts, novel MIPs might offer new avenues for biorecognition and isomeric separation techniques. The MIPs developed in this study may be used for bio-separations in conjunction with chromatographic techniques to separate amino acids, sugars, drugs, viruses and other bioactive compounds. In previous work K-12 activities have been developed. An interactive Website for K-12 will be developed in this project.