SGER: A novel approach to a biocompatible antibody-antigen recognition system using antigen imprinted polymers

摘要建议标题：SGER一种使用抗原印迹聚合物的生物相容抗体-抗原识别系统的新方法。建议编号：CTS-0640778首席研究员：彼得·科菲纳斯，研究所：马里兰大学学院公园这项探索性研究的学术价值和目标是利用PI实验室已经开发的技术将肿瘤特异性抗原(Ag)印记到聚合物支架中。由此产生的分子印迹聚合物(MIP)将包含抗原形状的空腔，这将引发特定的免疫系统反应，触发抗体的产生。这项工作的假设是，印迹过程将在聚合物基质中产生与目标抗体的形状和大小互补的空洞，导致该抗体与Ag-MIP特异和优先的特异性结合。Ag-MIP的识别能力将通过在竞争性和非竞争性亲和实验中测试不同同种类型抗体的亲和力来确定。拟议的研究涉及到开发新的技术，具有人类免疫系统中无与伦比的抗体-抗原识别能力，这可能在医学和生物技术领域产生深远的影响。这些技术将为创造革命性的检测和治疗疾病的技术铺平道路。银分子印迹聚合物可应用于亲和基质，用于分离纯化、生物传感器技术和诊断。所有针对癌症的免疫学方法背后的理念是，肿瘤细胞必须与正常细胞不同，并且这些差异必须被免疫系统检测到。肿瘤免疫学家试图利用其他物种产生的抗体来区分肿瘤和正常细胞；这种差异可以用于诊断、预后或治疗目的。这项研究中开发的针对MZ2-E肿瘤特异性抗原的聚合物，当注射到癌症患者身上时，可以引发免疫反应(抗体产生)，这可能有助于溶解(杀死)癌症肿瘤。由分子印迹蛋白制成的人造抗体也可用于诊断，检测血液样本中的抗原和抗体，分析血清以发现新的疾病标志物，以及环境和食品监测。在生物传感器技术中，人造抗体可以用来分析蛋白质的表达情况，并检测特定的蛋白质之间的相互作用。在这项工作中要开展的教育活动包括研究生辅导。

Abstract Proposal Title: SGER A novel approach to a biocompatible antibody-antigen recognition system using antigen imprinted polymers. Proposal Number: CTS-0640778 Principal Investigator: Peter Kofinas, Institution: University of Maryland College ParkThe intellectual merit and aim of this exploratory research is to imprint a tumor specific antigen (Ag) into a polymeric scaffold using techniques already developed in the PI's laboratory. The resulting molecularly imprinted polymers (MIPs) would contain antigen-shaped cavities, which would elicit specific immune system responses trigering the production of antibodies. The hypothesis for this work is that the imprinting process will create cavities within the polymer matrix that are complementary to the shape and size of the targeted antibody, resulting in the specific and preferential specific binding of that antibody to the Ag-MIP. The recognition ability of the Ag-MIP will be determined by testing the affinity for antibodies of different isotypes in competitive and non-competitive affinity experiments.The broader impacts of the proposed research relate to development of novel techonolgies with the unparalleled recognition capability of antibody-antigen recognition present in the human immune system, which could have profound effects within the field of medicine and biotechnology. Such technologies would pave the way for the creation of revolutionary tech- niques to detect and treat diseases. Ag-MIPs can be applied to affinity matrices for applications in separation and purification, biosensor technology and diagnostics. Underlying all immunological approaches to cancer is the idea that tumor cells must differ from normal cells, and that these differences must be detectable by the immune system. Tumor immunologists have tried to exploit this by using antibodies produced in another species to distinguish between tumor and normal cells; this difference can then be exploited for diagnostic, prognostic, or therapeutic purposes. The polymer developed in this research imprinted against the MZ2-E tumor-specific antigen could elicit an immune response (antibody production), when injected to cancer patients, which could help lyse (kill) the cancerous tumor. Artifical antibodies made from MIPs can also be used in diagnostics for the detection of antigens and antibodies in blood samples, for the profiling of sera to discover new disease markers, and for environment and food monitoring. In biosensor technology artificial antibodies can be used to profile protein expression and to detect specific protein-protein interactions. The education activities to be undertaken in this work include graduate student mentoring.