NIRT: Nanoscale Manipulation of Biological Entities using Magnetic Fluids and Fields

NIRT：使用磁流体和磁场对生物实体进行纳米级操控

• 批准号: 0304128
• 负责人: Patrick Doyle
• 金额: $ 100万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0304128&HistoricalAwards=false
• 关键词: NIRT; Nanoscale; Manipulation; Biological; Entities

PROPOSAL: 0304128 PI: Patrick Doyle, MITABSTRACT This proposal was received in response to Nanoscale Science and Engineering initiative, NSF 02-148, category NIRT. The proposed research program focuses on using magnetic fluids (based on magnetic nanoparticles) and fields to develop new methods of separation of biological particles such as cell fragments, DNA, viruses and inclusion bodies in bioMEMs devices and for controlling cell function. Process fluids will be seeded with nanoscopic (10-20 nm) magnetic particles suspended in a fluid phase to form magnetic fluids. Control of the spatial distribution of the nanoparticles will attained by manipulation of the direction and strength of the applied magnetic fields. Non-magnetic biological entities suspended in such a fluid would be subjected to magnetophoretic forces under a magnetic field gradient, and their migration rates will be determined by their size and morphology, which will permit separations based on density, size, and perhaps charge. In a related approach, magnetic beads will be aligned in an arroy of columns, and this array can serve as an effective sieving medium for the electrophoretic resolution of DNA fragments.The project is being funded by two programs within CTS: Particulate and Multiphase Processes and Fluid Dynamics and Hydraulics

提案：0304128 PI：Patrick Doyle，MITABSTRACT本提案是对NSF02-148，NIRT类纳米科学与工程倡议的响应。拟议的研究计划侧重于使用磁性流体(基于磁性纳米颗粒)和磁场来开发新的方法，以分离生物微机械设备中的细胞碎片、DNA、病毒和包涵体等生物颗粒，并控制细胞功能。工艺流体将被悬浮在流体相中的纳米(10-20 nm)磁性颗粒播撒，以形成磁性流体。控制纳米颗粒的空间分布将通过操纵外加磁场的方向和强度来实现。悬浮在这种流体中的非磁性生物实体将受到磁场梯度下的磁致伸缩力，它们的迁移率将由它们的大小和形态决定，这将允许基于密度、大小甚至电荷进行分离。在一种相关的方法中，磁珠将在柱子阵列中排列，该阵列可以作为有效的筛分介质用于DNA片段的电泳性分辨。该项目由CTS内的两个项目资助：颗粒和多相过程以及流体动力学和水力学