NIRT: Nanoscale Manipulation of Biological Entities using Magnetic Fluids and Fields

摘要该提案收到于纳米尺度科学与工程计划（NSF 02-148，类别NIRT）的响应。拟议的研究计划侧重于使用磁流体（基于磁性纳米颗粒）和领域，以开发生物机械设备中分离生物颗粒（如细胞片段、DNA、病毒和包涵体）和控制细胞功能的新方法。在工艺流体中加入纳米级（10-20纳米）磁性颗粒，悬浮在流体相中，形成磁性流体。通过控制外加磁场的方向和强度来控制纳米粒子的空间分布。悬浮在这种流体中的非磁性生物实体将在磁场梯度下受到磁泳力的影响，它们的迁移速率将由它们的大小和形态决定，这将允许基于密度、大小和可能的电荷进行分离。在一种相关的方法中，磁珠将在一列列中对齐，并且该阵列可以作为DNA片段电泳分辨率的有效筛选介质。该项目由CTS的两个项目资助：颗粒和多相过程以及流体动力学和水力学

PROPOSAL: 0304128 PI: Patrick Doyle, MITABSTRACT This proposal was received in response to Nanoscale Science and Engineering initiative, NSF 02-148, category NIRT. The proposed research program focuses on using magnetic fluids (based on magnetic nanoparticles) and fields to develop new methods of separation of biological particles such as cell fragments, DNA, viruses and inclusion bodies in bioMEMs devices and for controlling cell function. Process fluids will be seeded with nanoscopic (10-20 nm) magnetic particles suspended in a fluid phase to form magnetic fluids. Control of the spatial distribution of the nanoparticles will attained by manipulation of the direction and strength of the applied magnetic fields. Non-magnetic biological entities suspended in such a fluid would be subjected to magnetophoretic forces under a magnetic field gradient, and their migration rates will be determined by their size and morphology, which will permit separations based on density, size, and perhaps charge. In a related approach, magnetic beads will be aligned in an arroy of columns, and this array can serve as an effective sieving medium for the electrophoretic resolution of DNA fragments.The project is being funded by two programs within CTS: Particulate and Multiphase Processes and Fluid Dynamics and Hydraulics