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DISSERTATION RESEARCH: Testing the Mutation Models for the Evolution of Microsatellites: An Experimental and Simulation Approach

论文研究：测试微卫星进化的突变模型：实验和模拟方法

基本信息

批准号：
0308895
负责人：
Paul Sniegowski
金额：
$ 1.16万
依托单位：
University of Pennsylvania
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2003
资助国家：
美国
起止时间：
2003-06-01 至 2005-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0308895&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Testing Mutation Models

项目摘要

Microsatellites are stretches of DNA composed of small sequence units that are repeated many times in tandem. The number of repeats is highly mutable, and the appropriate model for the mutational processes affecting repeat length remains an unsolved problem. This research will use experimental evolution to address this problem. Twenty replicate populations of the yeast Saccharomyces cerevisiae will be evolved for 2000 generations and the variation that has accumulated at 6 microsatellite loci will be characterized. The results will be compared to predictions from computer simulation studies based on the three major mutational models that have been proposed. If none of these preexisting models is confirmed, then the extensive and novel data set obtained in this experiment will be employed to develop a more appropriate model.Microsatellites are present in the genomes of most organisms. Analysis of microsatellite variation is used to study such basic scientific questions as population sizes, genetic divergence between populations, and evolutionary relationships between species. At a more applied level, microsatellites are important tools in genome mapping in medical research and forensic analyses in legal proceedings. Interpretation of results in all of these areas typically requires assumptions about the mutational processes affecting microsatellite variation. The results of this project will thus have important implications in diverse areas of biology.

微卫星是由串联重复多次的小序列单元组成的DNA片段。重复序列的数量是高度可变的，影响重复序列长度的突变过程的适当模型仍然是一个未解决的问题。这项研究将使用实验进化来解决这个问题。20个复制群体的酵母酿酒酵母将进化2000代，并在6个微卫星位点上积累的变异将被表征。研究结果将与基于已提出的三种主要突变模型的计算机模拟研究的预测进行比较。如果这些先前存在的模型都没有得到证实，那么在这个实验中获得的广泛和新颖的数据集将被用来开发一个更合适的模型。微卫星变异分析被用来研究诸如种群大小、种群间遗传分化和物种间进化关系等基本科学问题。在更实用的层面上，微卫星是医学研究中的基因组绘图和法律的诉讼中的法医分析的重要工具。解释所有这些领域的结果通常需要假设影响微卫星变异的突变过程。因此，该项目的结果将对生物学的各个领域产生重要影响。