Capillary Electrophoresis in the Undergraduate Curriculum in Biochemistry and Forensic Chemistry

毛细管电泳在生物化学与法医化学本科课程中的应用

• 批准号: 0311080
• 负责人: Jenq-Kuen Huang
• 金额: $ 4.81万
• 依托单位: Western Illinois University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2005-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0311080&HistoricalAwards=false
• 关键词: Capillary; Electrophoresis; Undergraduate; Curriculum; Biochemistry

Chemistry (12) Capillary electrophoresis (CE) techniques have become widely accepted by the scientific community. To fully prepare students for careers in science or to pursuit advanced degree, we are including CE methodology into our undergraduate curriculum. Thin layer chromatography, SDS-polyacrylamide and agarose gel electrophoresis, and conventional liquid chromatography have been used for some time to separate biomolecules in our undergraduate biochemistry laboratories. With the acquisition of a versatile CE system that can be used in the analysis of many types of molecules, we are now adapting and implementing a specially designed set of experiments into our undergraduate biochemistry and forensic chemistry curriculum. The experiments and protocols are all adapted from educational and research journals, and NSF supported projects that have been fully tested. These selected experiments allow our students to perform qualitative and quantitative analysis of samples of biological, pharmaceutical, and forensic significance (e.g., protein, DNA, and drug analysis) by direct comparison of CE to other methods of instrumental separation. We are also developing collaborations between the departments of law enforcement and justice administration, biology, and chemistry on the use of CE methodology. After successful incorporation of CE in biochemistry and forensic chemistry courses as anticipated, we plan to expand the applications of the CE system to general, organic, analytical, physical, and inorganic chemistry courses. The CE system is also used in undergraduate research in our department. We plan to disseminate the outcome of this project through publications in appropriate journals, attending scientific meetings, and establishing a web site for the project.

化学毛细管电泳法(CE)已被科学界广泛接受。为了让学生为从事科学工作或攻读更高的学位做好充分准备，我们正在将CE方法论纳入我们的本科课程。在我们的本科生物化学实验室中，薄层色谱、十二烷基硫酸钠-聚丙烯酰胺和琼脂糖凝胶电泳法以及传统的液相色谱分离生物大分子已经有一段时间了。随着获得了可用于多种类型分子分析的多功能CE系统，我们现在正在将一套特别设计的实验调整和实施到我们的本科生物化学和法医化学课程中。这些实验和方案都是从教育和研究期刊上改编的，NSF资助的项目已经过全面测试。这些选定的实验使我们的学生能够通过将CE与其他仪器分离方法进行直接比较，对具有生物、药物和法医学意义的样品进行定性和定量分析(例如，蛋白质、DNA和药物分析)。我们还发展了执法和司法、生物和化学部门之间在CE方法学使用方面的合作。在成功将CE引入生物化学和法医化学课程后，我们计划将CE系统的应用扩展到普通、有机、分析、物理和无机化学课程。CE系统也在我系本科生科研中使用。我们计划通过在适当的期刊上发表文章、参加科学会议和为该项目建立一个网站来传播这一项目的成果。