Capillary Electrophoresis in Undergraduate Education

毛细管电泳在本科教育中的应用

• 批准号: 9552043
• 负责人: John Harwood
• 金额: $ 1.61万
• 依托单位: Tennessee Technological University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9552043&HistoricalAwards=false
• 关键词: Capillary; Electrophoresis; Undergraduate; Education

The purchase of a capillary electrophoresis (CE) instrument will enable undergraduate students to become familiar with state of the art electrophoresis methodology and with computerized data acquisition and instrument control. Experiments using the instrument will replace or greatly enhance experiments presently performed in two upper level chemistry courses, Instrumental Analysis and General Biochemistry. The instrument will also be used in undergraduate research. In Instrumental Analysis, commercial products will be analyzed for organic acid composition, demonstrating the usefulness of CE in the analysis of small molecules. This experiment will replace one in which ion chromatography is used to determine the inorganic ion composition of tapwater. In General Biochemistry, CE will initially be used to add two new experiments to the laboratory course: 1) enzymatic and chemical digestion of a protein followed by separation and quantitation by free zone CE and 2) a protein-substrate binding analysis by affinity CE techniques followed by mathematical treatment of the data by the method of Scatchard. These experiments will allow the students exposure to both additional conventional and computational biotechniques. It is likely that CE will assume increasing importance in the undergraduate teaching laboratory. We intend to share the results of our experiences both through journal publication and presentations at regional and national scientific meetings.

购买毛细管电泳（CE）仪器将使本科生熟悉最先进的电泳方法，以及计算机数据采集和仪器控制。使用仪器的实验将取代或大大加强目前在两个高级化学课程中进行的实验，仪器分析和普通生物化学。该仪器也将用于本科生的研究。在仪器分析中，将分析商业产品的有机酸组成，展示CE在小分子分析中的有用性。本实验将取代用离子色谱法测定自来水中无机离子组成的实验。在《普通生物化学》中，CE最初将用于在实验课程中增加两个新实验：1)蛋白质的酶解和化学消化，然后用自由区CE分离和定量；2)用亲和CE技术进行蛋白质-底物结合分析，然后用Scatchard方法对数据进行数学处理。这些实验将使学生接触到额外的传统和计算生物技术。在本科教学实验室中，CE的作用将越来越重要。我们打算通过期刊出版和在区域和国家科学会议上的发言来分享我们的经验成果。