Capillary Electrophoresis in the Undergraduate Curriculum

毛细管电泳在本科课程中的应用

• 批准号: 9552334
• 负责人: Frank Gomez
• 金额: $ 2.62万
• 依托单位: California State L A University Auxiliary Services Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9552334&HistoricalAwards=false
• 关键词: Capillary; Electrophoresis; Undergraduate; Curriculum

Through this project, the university is incorporating in its curriculum practical work based on capillary electrophoresis (CE), thus enabling students to gain proficiency in this recently developed technique. CE is destined to become an indispensable analytical separations method because of its unrivaled efficiency. The acquisition of this equipment significantly upgrades the undergraduate chemistry and biochemistry laboratory programs for B.S. chemistry and biochemistry majors and B.A. chemistry majors. The experiments included in the analytical chemistry course illustrate the complementary nature of CE to HPLC by demonstrating its utility where liquid chromatography fails. The experiments included in the biochemistry laboratory courses illustrate the utility of CE in the area of protein analysis and protein-ligand binding. An automated unit permits students to develop awareness of the greatly increased workload achievable with a state-of-the-art instrument. It also compels students to revise their notions concerning the planning and design of experiments in order to exploit fully the productive capacity of a computer-controlled instrument. These curricular changes enable the university to maintain its excellent record of training undergraduates, particularly minorities and women, for research careers in the chemical and biological sciences.

通过这个项目，该大学在课程中加入了基于毛细管电泳法(CE)的实践工作，从而使学生能够熟练掌握这项最近开发的技术。毛细管电泳法以其无与伦比的高效性，注定成为一种不可或缺的分析分离方法。该设备的购买大大提升了本科生化学和生物化学专业以及化学学士专业的本科化学和生物化学实验室项目。包括在分析化学课程中的实验通过证明在液相色谱学失败的情况下CE的效用来说明CE对高效液相色谱的补充性质。生物化学实验室课程中包含的实验说明了CE在蛋白质分析和蛋白质配基结合领域的应用。自动化单元使学生意识到使用最先进的仪器可以极大地增加工作量。它还迫使学生改变他们对实验计划和设计的观念，以充分发挥计算机控制仪器的生产能力。这些课程改革使该大学能够保持其培养本科生，特别是少数民族和女性本科生从事化学和生物科学研究事业的良好记录。