Capillary Electrophoresis in the Undergraduate Curriculum in Chemistry and Biological Chemistry

毛细管电泳在化学与生物化学本科课程中的应用

• 批准号: 9850730
• 负责人: Thomas Wenzel
• 金额: $ 2.18万
• 依托单位: Bates College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9850730&HistoricalAwards=false
• 关键词: Capillary; Electrophoresis; Undergraduate; Curriculum; Chemistry

Capillary electrophoresis will be incorporated into the undergraduate curriculum in chemistry and biochemistry at Bates College. Electrophoretic techniques are of growing importance in chemical analysis, in large part because of the increase in work at the interface of chemistry and biology. Students at the undergraduate level should therefore be introduced to the theoretical and practical aspects of electrophoresis, and particularly capillary electrophoresis. The instrumentation will be utilized in undergraduate laboratories associated with courses in analytical and biological chemistry. The lab experiences in these courses are project-based, so the experiments that will be added to the curriculum utilizing capillary electrophoresis are ambitious in scope. Examples of the types of projects that will be undertaken include the analysis of neuropeptides in brain tissue, hemoglobin variants in blood, DNA restriction fragment analysis of human DNA, and protein mapping to confirm the effectiveness of protein modification through site selected mutagenesis. Capillary electrophoresis will complement the methods, techniques and experiences that are already provided in undergraduate courses in analytical and biological chemistry. Capillary electrophoresis equipment will also be beneficial to students conducting research projects at the interface of biology and chemistry.

毛细管电泳将被纳入贝茨学院的化学和生物化学本科课程。 电泳技术在化学分析中的重要性越来越大，这在很大程度上是因为化学和生物学界面工作的增加。因此，本科阶段的学生应了解电泳的理论和实践方面，特别是毛细管电泳。 该仪器将用于与分析和生物化学课程相关的本科实验室。在这些课程的实验室经验是基于项目的，所以将被添加到利用毛细管电泳课程的实验是雄心勃勃的范围。将进行的项目类型的例子包括分析脑组织中的神经肽、血液中的血红蛋白变体、人类DNA的DNA限制性片段分析和蛋白质绘图，以确认通过定点诱变进行蛋白质修饰的有效性。毛细管电泳将补充分析和生物化学本科课程中已经提供的方法，技术和经验。毛细管电泳设备也将有利于学生在生物和化学的界面进行研究项目。