Capillary Electrophoresis in the Undergraduate Curriculum in Chemistry and Biological Chemistry

毛细管电泳将被纳入贝茨学院的化学和生物化学本科课程。电泳技术在化学分析中越来越重要，这在很大程度上是因为化学和生物界面上工作的增加。因此，应该向本科生介绍电泳学的理论和实践方面，特别是毛细管电泳学。该仪器将用于与分析和生物化学课程相关的本科实验室。这些课程中的实验经验是基于项目的，因此将利用毛细管电泳法添加到课程中的实验在范围上是雄心勃勃的。将开展的项目类型包括分析脑组织中的神经肽、血液中的血红蛋白变体、人类DNA的DNA限制性片段分析，以及通过定点突变确认蛋白质修饰的有效性的蛋白质图谱。毛细管电泳会补充已经在分析和生物化学本科课程中提供的方法、技术和经验。毛细管电泳仪也将有利于学生在生物和化学的交界处进行研究项目。

Capillary electrophoresis will be incorporated into the undergraduate curriculum in chemistry and biochemistry at Bates College. Electrophoretic techniques are of growing importance in chemical analysis, in large part because of the increase in work at the interface of chemistry and biology. Students at the undergraduate level should therefore be introduced to the theoretical and practical aspects of electrophoresis, and particularly capillary electrophoresis. The instrumentation will be utilized in undergraduate laboratories associated with courses in analytical and biological chemistry. The lab experiences in these courses are project-based, so the experiments that will be added to the curriculum utilizing capillary electrophoresis are ambitious in scope. Examples of the types of projects that will be undertaken include the analysis of neuropeptides in brain tissue, hemoglobin variants in blood, DNA restriction fragment analysis of human DNA, and protein mapping to confirm the effectiveness of protein modification through site selected mutagenesis. Capillary electrophoresis will complement the methods, techniques and experiences that are already provided in undergraduate courses in analytical and biological chemistry. Capillary electrophoresis equipment will also be beneficial to students conducting research projects at the interface of biology and chemistry.