Functional Genomic Approach to Signal Transduction and Innate Immunity in Shrimp

虾信号转导和先天免疫的功能基因组方法

• 批准号: 0315393
• 负责人: Gregory Warr
• 金额: --
• 依托单位: Medical University of South Carolina
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0315393&HistoricalAwards=false
• 关键词: Functional; Genomic; Approach; Signal; Transduction

This research is focused on understanding the immune response of a crustacean, the marine shrimp, Litopenaeus vannamei to virus infections. Crustacea are essential components of the marine environment and marine shrimp are of commercial significance, especially since these animals are widely aquacultured. These animals are highly susceptible to numerous viruses, including the Taura Syndrome Virus and White Spot Syndrome Virus, and these have devastating impacts on aquaculture and possible unknown consequences for the health of the marine environment. This research addresses the hypothesis that 2 shrimp molecules (STAT and IkK, which are families of immune function proteins) are components of the molecular machinery by which shrimp recognize and respond to viral infection. There are two Specific Aims. 1) To reduce, by RNA interference methods, the expression of STAT and IkK, and establish the effect of this reduced expression on shrimp resistance to viral infection. 2) To identify the genes regulated by STAT and IkK that are important in viral immunity. "Functional genomics" approaches will be employed, using DNA microarrays to measure the levels of expression of approximately 3,000 shrimp genes simultaneously. Bioinformatic analyses of the resulting data will identify genes participating in the host response to viral infection, and the role of STAT and IkK.This research will increase our understanding of marine invertebrate immunity, and assist in the management of marine ecosystems and aquaculture resources. The broader impacts of this work include: 1) the dissemination of information on marine organisms and the marine environment to professionals and the public, through coordination with the South Carolina Department of Natural Resources and 2) the education and training of graduate students who will work on the project. In addition, through participation in summer research exposure programs, undergraduate students will have the opportunity to work on this project and to gain experience in research.

本研究旨在了解南美白对虾这种甲壳类动物对病毒感染的免疫反应。甲壳类是海洋环境的重要组成部分，海洋对虾具有商业意义，特别是因为这些动物被广泛水产养殖。这些动物对多种病毒高度敏感，包括陶拉综合症病毒和白斑综合症病毒，这些病毒对水产养殖造成毁灭性影响，并可能对海洋环境健康造成未知后果。这项研究提出的假设是，两个虾分子(STAT和IKK，这两个免疫功能蛋白家族)是虾识别和响应病毒感染的分子机制的组成部分。有两个具体目标。1)通过RNA干扰方法降低STAT和IKK的表达，并建立这种降低的表达对对虾抗病毒侵染的影响。2)寻找在病毒免疫中起重要作用的STAT和IKK调控基因。将采用“功能基因组学”方法，使用DNA微阵列同时测量大约3000个虾基因的表达水平。对所得数据的生物信息学分析将确定参与宿主对病毒感染的反应的基因，以及STAT和IkK的作用。这一研究将增加我们对海洋无脊椎动物免疫的了解，并有助于海洋生态系统和水产养殖资源的管理。这项工作的更广泛影响包括：1)通过与南卡罗来纳州自然资源部的协调，向专业人员和公众传播有关海洋生物和海洋环境的信息；2)对将参与该项目的研究生进行教育和培训。此外，通过参与暑期研究暴露计划，本科生将有机会参与这个项目并获得研究经验。