Functional analysis of chromatin dynamics during aging by genomic approach

染色质结构在基因表达中起着基础性的作用，随着细胞条件和环境的变化，染色质结构可以被组织起来。在本研究中，我们试图了解染色质结构变化在衰老过程中的分子机制和作用。应用CHIP-SEQ方法对哺乳动物脑内染色质因子的结合部位进行鉴定。我们的结果表明，在大脑可塑性增强的关键时期，必需的转录因子OTX2和高阶染色质结构的调节者粘附素共同作用于基因调控。

Chromatin structure has fundamental roles in gene expression, which could bere-organized as conditions and circumstances of the cells change. In this study, wehave tried to understand the molecular mechanism and the roles of the structuralchanges in chromatin during aging. ChIP-seq method was applied to identify thebinding sites of chromatin factors throughout the genome in mammalian brain. Ourresults indicated that during the critical period in which the plasticity of the brainincreases, Otx2, the essential transcription factor, and cohesin, the regulator of higherorder chromatin structure, act cooperatively for gene regulation.