Functional analysis of chromatin dynamics during aging by genomic approach

通过基因组方法对衰老过程中染色质动态进行功能分析

• 批准号: 22710183
• 负责人: SAKAI Akiko
• 金额: $ 3.91万
• 依托单位: Niigata University (2011-2012)Tokyo Institute of Technology (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22710183/
• 关键词: ゲノム; ChIP-seq; 転写制御; クロマチン; 発現制御

Chromatin structure has fundamental roles in gene expression, which could bere-organized as conditions and circumstances of the cells change. In this study, wehave tried to understand the molecular mechanism and the roles of the structuralchanges in chromatin during aging. ChIP-seq method was applied to identify thebinding sites of chromatin factors throughout the genome in mammalian brain. Ourresults indicated that during the critical period in which the plasticity of the brainincreases, Otx2, the essential transcription factor, and cohesin, the regulator of higherorder chromatin structure, act cooperatively for gene regulation.

染色质结构在基因表达中起着重要作用，随着细胞条件和环境的改变，基因表达会发生变化。本研究试图了解衰老过程中染色质结构变化的分子机制和作用。应用ChIP-seq方法对哺乳动物脑内染色质因子的结合位点进行了研究。我们的研究结果表明，在大脑可塑性增加的关键时期，Otx 2，一个重要的转录因子，和cohesin，一个高级染色质结构的调节器，协同作用的基因调控。

项目成果

経験依存的な神経回路の可塑性に関わるクロマチン動態の解析

与经验依赖性神经回路可塑性相关的染色质动力学分析

• 发表时间: 2012
• 作者: 酒井晶子;桑野良三;杉山清佳
• 通讯作者: 杉山清佳

Chromatin dynamics during the critical period of ocular dominance plasticity in mouse visual cortex

小鼠视觉皮层眼优势可塑性关键期的染色质动态

• 发表时间: 2012
• 作者: Sato S;Yagi S;Arai Y;Hirabayashi K;Hattori N;Iwatani M;Okita K;Ohgane J;Tanaka S;Wakayama T;Yamanaka S;Shiota K;三浦史仁;Akiko Sakai
• 通讯作者: Akiko Sakai

Chromatin dynamics duringthe critical period of ocular dominanceplasticity in mouse visual cortex

小鼠视觉皮层眼优势可塑性关键期的染色质动态

• 发表时间: 2012
• 作者: 江戸翔;小泉圭吾;小泉圭吾;小泉圭吾;小泉圭吾;小泉圭吾;Akiko Sakai
• 通讯作者: Akiko Sakai

臨界期におけるマウス視覚野のクロマチン動態の解析

关键期小鼠视觉皮层染色质动态分析

• 发表时间: 2012
• 作者: 酒井晶子;中戸隆一郎;桑野良三;白髭克彦;杉山清佳
• 通讯作者: 杉山清佳