Peptide Conformation Studies with Vibrational Spectroscopy and Site Selective Isotopic Labeling

利用振动光谱和位点选择性同位素标记进行肽构象研究

• 批准号: 0316014
• 负责人: Timothy Keiderling
• 金额: $ 37.7万
• 依托单位: University of Illinois at Chicago
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0316014&HistoricalAwards=false
• 关键词: Peptide; Conformation; Studies; Vibrational; Spectroscopy

This project, funded by the Experimental Physical Chemistry Program of the Chemistry Division, deals with peptide conformations, energetics, and dynamics of protein structure and folding. Most oligopeptides are quite flexible and thereby inhibit conventional structural analyses, especially under conditions where one structure is changing into another, such as folding. To overcome these difficulties Keiderling will conduct a research program to place the interpretation of infrared (IR), Raman, vibrational circular dichroism (VCD) and Raman optical activity, as well as time-dependent Raman and IR spectra experiments on a solid foundation. To this end he will (a) employ isotopically labeled systems to give the vibrational spectra resolved, site-specific character, and (b) develop a more reliable interpretative basis for the vibrational spectral intensity band shapes measured in peptides. The experimental efforts will be aided by DFT augmented Gaussian 98 calculations to obtain spectral parameters for computational modeling of the spectra. Most experimental and computational efforts will focus on beta-hairpin and beta-sheet modeling. Peptides of different lengths with various beta-turn stabilizing residues will be studied while thermally unfolded and refolded. The overall goal of the work is the development of enhanced structural biology tools, both experimental and theoretical, and obtain a basic understanding of folding energetics, capable of addressing broad biophysical needs. This research will be conducted with students and postdoctoral research associates. They will thereby acquire skills and training in one of the fast developing forefront areas of contemporary bio-physical chemistry, in preparation for advanced studies or employment in the scientific/technological sectors of our economy.

该项目由化学部实验物理化学计划资助，涉及肽构象，能量学以及蛋白质结构和折叠的动力学。 大多数寡肽是相当灵活的，从而抑制常规的结构分析，特别是在一种结构改变成另一种结构的条件下，如折叠。 为了克服这些困难，Keiderling将进行一项研究计划，将红外（IR），拉曼，振动圆二色性（VCD）和拉曼光学活性的解释，以及时间依赖的拉曼和红外光谱实验的坚实基础。 为此，他将（a）采用同位素标记的系统来给出振动光谱的分辨、位点特异性特征，（B）为肽中测量的振动光谱强度带形建立更可靠的解释基础。 实验的努力将有助于DFT增强高斯98计算，以获得光谱的计算建模的光谱参数。 大多数实验和计算工作将集中在β-发夹和β-折叠建模。 将研究具有各种β-转角稳定残基的不同长度的肽，同时热解折叠和重折叠。 这项工作的总体目标是开发增强的结构生物学工具，包括实验和理论，并对折叠能量学有基本的了解，能够满足广泛的生物物理需求。 这项研究将与学生和博士后研究助理进行。 因此，他们将获得当代生物物理化学快速发展的前沿领域之一的技能和培训，为我们经济的科学/技术部门的高级研究或就业做准备。