Deciphering Mechanisms of Speciation Among Host-Symbiont Populations

破译宿主共生群体之间的物种形成机制

• 批准号: 0316516
• 负责人: Michele Nishiguchi
• 金额: $ 38.7万
• 依托单位: New Mexico State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0316516&HistoricalAwards=false
• 关键词: Deciphering; Mechanisms; Speciation; Among; Host

The mutualistic association between sepiolid squids (Mollusca: Cephalopoda) and their luminous bacterial symbionts (Genus Vibrio) provides a versatile model system to study population biology and speciation among animal-bacterial partnerships. Although the squid light organs are open to the seawater, only two Vibrio species stay inside the organ, indicating adaptation and evolution of specificity. Using state-of-the-art techniques involving fluorescent microscopy, molecular biology, and gene expression, this project will examine whether environmental processes or the squid hosts determine the architecture of these interactions. Central to this study will be determining how different the squid-Vibrio pairs are among geographically isolated populations. A key element of this proposal is the several international and collaborative efforts that have been established by the Nishiguchi laboratory for this work. Results from this proposal will be shared (through publications, meetings, web sites, courses) with both the research and public communities. This study will provide unique research opportunities for students, building on programs for under-represented minorities at New Mexico State University. NMSU is the only Carnegie I research institution ranked by the National Science Foundation as one that serves significant numbers of two ethnic minority student populations, Hispanics and Native Americans. This research will lead to a better understanding of speciation and evolution of bacterial-animal relationships and provide information about the transmission of bacteria in the environment.

乌贼（软体动物门：头足目）和发光细菌共生体（弧菌属）之间的互惠关系提供了一个通用的模型系统，研究种群生物学和物种形成之间的动物-细菌的伙伴关系。 虽然鱿鱼的发光器官对海水开放，但只有两种弧菌留在器官内，表明适应和进化的特异性。 使用涉及荧光显微镜，分子生物学和基因表达的最先进技术，该项目将研究环境过程或鱿鱼宿主是否决定了这些相互作用的结构。 这项研究的核心将是确定在地理上孤立的人群中鱿鱼-弧菌对的差异。 这项提议的一个关键因素是西口实验室为这项工作建立的几项国际合作努力。这项建议的成果将（通过出版物、会议、网站、课程）与研究界和公众分享。 这项研究将为学生提供独特的研究机会，建立在新墨西哥州州立大学代表性不足的少数民族的计划。NMSU是唯一一个被国家科学基金会列为卡内基I研究机构，为两个少数民族学生群体，西班牙裔和美洲原住民提供服务。 这项研究将有助于更好地了解细菌-动物关系的物种形成和进化，并提供有关细菌在环境中传播的信息。