Acquisition of a Chemistry/Biology Biomolecular Imaging Center

收购化学/生物学生物分子成像中心

• 批准号: 0320801
• 负责人: Rebecca Alexander
• 金额: $ 4.23万
• 依托单位: Wake Forest University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0320801&HistoricalAwards=false
• 关键词: Acquisition; Chemistry; Biology; Biomolecular; Imaging

A grant has been awarded to Wake Forest University under the direction of Dr. Rebecca Alexander and Dr. Bernard Brown II. This grant will help to establish an interdepartmental, multi-user, biomolecular imaging facility on the Reynolda campus of Wake Forest University. With an increasing number of research programs at the interface of chemistry and biology, Wake Forest has been building its instrumental infrastructure. In particular, researchers needed imaging equipment to analyze data rapidly and with increased sensitivity. This grant will support the purchase of a chemiluminescent/fluorescent imager and associated peripherals to supplement a recently acquired phosphorimager. The imager will be used for image capture of ethidium bromide-stained DNA gels; dye or silver-stained protein gels; chemiluminescent blotting applications; colony counting of agar plates; and other applications. The initial group of seven major users includes four faculty in the Chemistry Department and three in the Biology Department. Each investigator supervises undergraduate and graduate research projects in addition to teaching undergraduate and graduate courses. Four are junior faculty within the first four years of appointment, and this imaging facility is expected to increase their research productivity and improve the quality of data submitted to funding agencies and for publication. In addition to the major users included in the proposal, others in the Biology, Chemistry, and Physics Departments are likely to take advantage of the imaging facility once it is fully operational. It is estimated that as many as 80 undergraduates and graduate students will access the facility during the funding period. Funding of this proposal will have significant impact on teaching and outreach at Wake Forest University. In particular, WFU is pursuing approval for an undergraduate biochemistry major, including a required biochemistry laboratory and independent research projects. Access to contemporary imaging equipment will be vital to this training. Women and minorities are well-represented in both the undergraduate and graduate programs in the WFU Chemistry and Biology Departments, and their familiarity with the instrumentation described here will enhance their training and future career opportunities. In addition, science faculty from Salem College, a women's college in Winston-Salem, have expressed interest in demonstrating various imaging techniques to students, and equipment purchased through this award will be used in this outreach capacity. Wake Forest researchers will also use the instrumentation in an ongoing collaborative project with faculty and students at Winston-Salem State University, a historically Black university. Finally, this equipment will be available for demonstration to middle and high school teachers in the local public school district as a part of wider faculty outreach efforts.

在丽贝卡亚历山大博士和伯纳德布朗二世博士的指导下，维克森林大学获得了一笔赠款。 这笔赠款将有助于建立一个跨部门，多用户，生物分子成像设施的维克森林大学校园内。 随着越来越多的研究项目在化学和生物学的接口，维克森林一直在建设其仪器基础设施。 特别是，研究人员需要成像设备来快速分析数据，并提高灵敏度。 这笔赠款将支持购买荧光/荧光成像仪和相关的外围设备，以补充最近购买的磷光成像仪。 成像仪将用于溴化乙锭染色的DNA凝胶的图像捕获;染料或银染色的蛋白质凝胶;荧光印迹应用;琼脂平板的菌落计数;以及其他应用。 最初的七个主要用户包括化学系的四名教师和生物系的三名教师。 每个研究员监督本科和研究生的研究项目，除了教本科和研究生课程。 四个是初级教师在任命的头四年内，这一成像设施预计将提高他们的研究生产力，提高提交给资助机构和出版的数据质量。 除了提案中包括的主要用户外，生物、化学和物理系的其他用户也可能在成像设施完全投入使用后利用该设施。 据估计，多达80名本科生和研究生将在资助期间使用该设施。 这项提案的资助将对维克森林大学的教学和推广产生重大影响。 特别是，WFU正在寻求批准本科生生物化学专业，包括所需的生物化学实验室和独立的研究项目。 获得现代成像设备对这一培训至关重要。妇女和少数民族在WFU化学和生物系的本科和研究生课程中都有很好的代表性，他们对这里描述的仪器的熟悉程度将提高他们的培训和未来的职业机会。 此外，温斯顿-塞勒姆的一所女子学院-塞勒姆学院的科学教师表示有兴趣向学生演示各种成像技术，通过该奖项购买的设备将用于这一推广活动。 维克森林的研究人员还将在一个正在进行的合作项目中使用仪器与教师和学生在温斯顿-塞勒姆州立大学，一个历史上的黑人大学。 最后，作为更广泛的教师外联工作的一部分，这些设备将可用于向当地公立学区的初中和高中教师演示。