MRI: Acquisition of an X-band Electron Paramagnetic Resonance (EPR) Spectrometer to Enhance the Infrastructure for Research in Redox Processes in Chemistry and Biology

该奖项由主要研究仪器（MRI）和化学研究仪器（CRIF）计划支持。来自伊利诺伊大学厄巴纳-香槟分校（UIUC）的Alison Fout教授及其同事Robert Gennis，Yi Lu和Dean Olson获得了X波段电子顺磁共振（EPR）光谱仪。该仪器允许在各种领域进行研究，例如那些对生物相关和环境重要物种的行为提供见解的领域。一般来说，EPR光谱仪产生分子和固态材料的几何和电子结构的详细信息。它也可用于获得有关参与有价值的化学转化以及病理性肿瘤生长起始的短寿命、高反应性物种的寿命的信息。这些研究影响了多个领域，从无机和有机分子的合成到新固态材料的开发，再到磁性、生物和医学化合物。该仪器用于培养下一代的学生研究人员都在UIUC和附近的机构，东伊利诺伊大学。这EPR光谱仪加强各级研究和教育。通过研究转氢酶的机制，调整单个蛋白质中金属中心的氧化还原电位和开发具有用于碳-X键形成的强场配体的钴（I）催化剂，它可能会影响将质子泵与氢化物转移联系起来的研究。该仪器还用于研究由生物激发的复合物制备的用于氧阴离子还原的化合物。 bc 1络合物中的电子转移和泛醌的五个催化位点的研究也在考虑之中。光谱仪有利于氧化还原酶的研究，推进化学气相沉积（CVD），并探索ROS敏感酶的氧化应激。此外，EPR还用于研究调节单个蛋白质中金属中心的氧化还原电位和表征P450催化的中间状态。

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation(CRIF) Programs. Professor Alison Fout from University of Illinois Urbana-Champaign (UIUC) and colleagues Robert Gennis, Yi Lu and Dean Olson have acquired an X-band electron paramagnetic resonance (EPR) spectrometer. This instrument allows research in a variety of fields such as those that provide insight on the behavior of biologically-relevant and environmentally-important species. In general, an EPR spectrometer yields detailed information on the geometric and electronic structures of molecular and solid state materials. It may also be used to obtain information about the lifetimes of short-lived, highly reactive species involved in valuable chemical transformations as well as the initiation of pathological tumor growth. These studies impact several areas, from the synthesis of inorganic and organic molecules to the development of new solid state materials to compounds of magnetic and biological and medical interest. The instrument serves to train the next generation of student researchers both at UIUC and the nearby institution, Eastern Illinois University.This EPR spectrometer enhances research and education at all levels. It may impact studies linking proton pumping to hydride transfer by studying the mechanism of transhydrogenase, tuning redox potentials of metal centers in a single protein and developing cobalt(I) catalysts featuring strong-field ligands for Carbon-X bond formation. The instrumentation is also used in the studies of compounds prepared by bio-inspired complexes for oxyanion reduction. Electron transfer in the bc1 complex and investigations of the five catalytic sites of ubisemiquinones are also under consideration. The spectrometer benefits investigations of redox enzymes, advances chemical vapor deposition (CVD), and explores oxidative stress in ROS-sensitive enzymes. In addition, the EPR is used for studies tuning redox potentials of metal centers in a single protein and characterizing intermediate states in P450 catalysis.