MRI: Acquisition of an LC-MS/MS to Enable Research and Training for Chemistry and Biology Students at SUNY Cortland

从化学主要研究仪器计划的这一奖项，教授杰弗里沃纳从纽约州立大学科特兰和同事克里斯塔查特菲尔德，特伦斯菲茨杰拉德，帕特里夏康克林和弗朗西斯罗西将获得一个线性离子阱质谱仪与集成液相色谱分离（LC/MS）。该提案旨在加强各级的研究和教育，特别是在以下领域：（a）利用蛋白质组学时间序列分析优化生物反应器中的开放微生物群落，生产碳链延长的商品化学品;（B）确定可用于减少与昆虫有关的作物损害的幼虫踪迹信息素;（c）阐明涉及嗜肺军团菌的环境生物膜形成的生理机制;和（d）确定VTC 3在植物中抗坏血酸调节中的作用。是用于识别和表征嵌入在复杂基质中的少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。具有串联能力的仪器通过进一步碎裂光谱仪中产生的离子提供额外的结构鉴定能力。作为一个主要的本科院校，纽约州立大学科特兰的教师拥抱本科研究经验作为他们的研究活动的最重要的成果之一。拟议的工具将直接影响至少28名学生在三年项目期间的研究经验，并在未来几年更多。增强校园能力的额外协同效益也将提高外部资金的竞争力，进一步促进和提高本科生的研究经验。本科研究人员将前往高需求学区与高中生分享他们的经验。他们对科学探究的真实叙述将激励未来的科学家们去做一个大学生可以做的前沿研究。最终，这些高中联系的目标是提高进入科学和技术领域的学生的多样性。

With this award from the Chemistry Major Research Instrumentation Program, Professor Jeffrey Werner from SUNY Cortland and colleagues Christa Chatfield, Terrence Fitzgerald, Patricia Conklin and Francis Rossi will acquire a linear ion trap mass spectrometer with integrated liquid chromatography separation (LC/MS). The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) use of proteomics time series analysis to optimize open microbial communities in a bioreactor producing extended carbon chain commodity chemicals; (b) identification of larval trail pheromones that could be exploited to reduce insect-related crop damage; (c) elucidation of the physiological mechanisms of environmental biofilm formation involving Legionella pneumophila; and (d) determination of the role of VTC3 in the regulation of ascorbic acid in plants.Mass Spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with tandem capability provides additional structural identification power through further fragmentation of ions produced in the spectrometer. As a primarily undergraduate institution, SUNY Cortland's faculty embraces undergraduate research experiences as one of the most important outcomes of their research activities. The proposed instrument will directly impact the research experiences of at least 28 students within the three-year project period, and many more in years to come. The added synergistic benefit of enhanced capabilities on campus will also increase competitiveness for external funding, further facilitating and enhancing undergraduate research experiences. Undergraduate researchers will travel to high-needs school districts to share their experiences with high school students. Their authentic narratives of scientific inquiry will inspire future scientists as to the leading-edge research one can do as a college student. Ultimately, the goal of these high school connections is to enhance the diversity of students entering science and technology fields.