Development of a Multiphoton Fluorescence Microscope for Time-Resolved and Super-Resolution Studies in Chemistry and Biology

开发用于化学和生物学时间分辨和超分辨率研究的多光子荧光显微镜

• 批准号: 0321232
• 负责人: Norbert Scherer
• 金额: $ 62.8万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321232&HistoricalAwards=false
• 关键词: Development; Multiphoton; Fluorescence; Microscope; Time

With support from the Major Research Instrumentation (MRI) Program, Norbert F. Scherer in the Chemistry Department at the University of Chicago will develop a multiphoton fluorescence microscope for time-resolved and super-resolution studies in chemistry and biology. The goals of this development project are a) to develop a non-linear optical microscope capable of sub-diffraction limit imaging by stimulated emission depletion (STED); b) to use fluctuation spectroscopy to determine local molecular concentrations in few-attoliter volumes and to establish lifetime imaging; and c) to investigate chemical reaction dynamics in very small volumes in heterogeneous materials via time-resolved STED. The PI will develop a user-friendly instrument capable of studying dynamics on a timescale from femtoseconds to seconds; in addition, the instrument will have 3-D imaging capabilities. These studies will bring state-of-the-art fluorescence microscopy techniques to the United States biological research community. In particular, the payoff for biological research will be quite substantial if superresolution can be demonstrated for a variety of fluorophore systems.

在重大研究仪器(MRI)计划的支持下，芝加哥大学化学系的Norbert F.Scherer将开发一种多光子荧光显微镜，用于化学和生物学中的时间分辨和超分辨率研究。这一开发项目的目标是a)开发一种能够通过受激辐射耗竭(STED)进行亚衍射极限成像的非线性光学显微镜；b)使用涨落光谱来确定少数阿托体积内的局部分子浓度并建立寿命成像；以及c)通过时间分辨STED来研究非均质材料中非常小体积的化学反应动力学。PI将开发一种用户友好的仪器，能够在从飞秒到几秒的时间尺度上研究动力学；此外，该仪器将具有3D成像能力。这些研究将为美国生物研究界带来最先进的荧光显微镜技术。特别是，如果能够证明各种荧光团系统的超分辨率，那么生物研究的回报将是相当可观的。