Acquisition of a High Capacity Genotyping Facility for Diverse Biological Applications

在 Paul Keim 博士的指导下，北亚利桑那大学获得了一笔拨款，用于购买高容量、高分辨率 DNA 基因分型和测序设施的核心仪器。遗传和基因组分析的力量已在人类和一些选定的模型基因组中得到充分证明，但如果这种技术易于使用，许多其他不同的生物物种和问题也将同样受益。北亚利桑那大学的研究人员致力于解决各种遗传和基因组问题，这些问题需要最先进的高容量 DNA 分析设施。这些仪器将能够进行中等规模的 DNA 测序（例如，小细菌基因组或多个样本中的单个基因座），但更重要的是，它们将通过 PCR 片段大小（即 DNA 指纹识别）提供高容量基因型分析。自动荧光检测 DNA 测序仪将用于确定与微卫星位点相关的 PCR 片段大小、检测 SNP、执行 AFLP 和 DNA 测序。中等规模的 DNA 测序能力对于通过靶向基因测序或小型基因组/质粒序列测定进行的分子系统学非常有价值。目前的赠款将用于建立环境基因组学和遗传学（EnGGEN）的多用户设施。 EnGGEN 将成为一所大学和区域资源中心，为来自区域学术、政府和私人机构的不同用户群提供分子遗传学分析和培训。已经致力于该设施的用户正在研究植物进化、生态群落遗传学、保护遗传学、微生物多样性和分子流行病学。 EnGGEN 将为梅里亚姆鲍威尔环境研究中心 (MPCER) 和梅里亚姆鲍威尔研究站提供补充设施，其使命是支持和促进公共和私营部门环境科学家、土地管理者和教育工作者之间的合作。他们的多元化成员将提供我们提议的环境遗传学设施的立即使用，并促进广泛的研究界的使用。为了促进整个大学和区域研究界（例如林务局、国家公园管理局、TNC、USGS）使用该设施，我们制定了一项研讨会、课程作业、实验室实践使用和奖学金机会的计划。这将有助于招募由于缺乏熟悉度和/或设施而通常不会在研究中使用遗传学或基因组分析的科学家。我们还将通过赞助研究生、本科生和 K-12 学生的讲习班、实验室课程和研讨会来支持大学课程活动。 NAU 拥有文化丰富的学生群体，主要代表 42 个美洲原住民部落和西班牙裔。我们将通过就地项目来指导这些学生，包括 NSF 资助的大学环境生物学指导 (UMEB) 拨款和针对本科生研究的 NAU 拨款计划。

A grant has been awarded to Northern Arizona University under the direction of Dr. Paul Keim to purchase core instrumentation for a high-capacity, high-resolution DNA genotyping and sequencing facility. The power of genetic and genomic analysis has been well demonstrated in the human and a few selected model genomes, but many other diverse biological species and problems would benefit equally if such technology were readily available. Researchers at Northern Arizona University are involved in diverse genetic and genomic problems that require a state-of-the-art, high capacity facility for DNA analyses. These instruments will be capable of moderate scale DNA sequencing (e.g. a small bacterial genome or single locus across many samples), but more importantly they will provide high capacity genotype analysis through PCR fragment sizing (i.e., DNA fingerprinting). An automated fluorescent detecting DNA sequencer will be used to size PCR fragments associated with microsatellite loci, detect SNPs, perform AFLP, and DNA sequencing. Moderate scale DNA sequencing capacity is valuable for molecular systematics by targeted gene sequencing, or small genome/plasmid sequence determination. The current grant will result in the establishment of a multi-user facility for ENvironmental Genomics and GENetics (EnGGEN). EnGGEN will be a university and regional resource center to provide molecular genetic analyses and training for a diverse user base from regional academic, government and private institutions. Users already committed to the facility are studying plant evolution, ecological community genetics, conservation genetics, microbial diversity, and molecular epidemiology. EnGGEN will provide a complementary facility to the Merriam Powell Center for Environmental Research (MPCER) and the Merriam-Powell Research Station whose missions are to support and foster collaborations among environmental scientists, land-managers, and educators from public and private sectors. Their diverse membership will provide immediate use of our proposed environmental genetics facility and promote its use by a broad research community.To promote the use of this facility throughout the University and regional research community (e.g., Forest Service, National Park Service, TNC, USGS), we have developed a plan of seminars, coursework, hands-on laboratory use, and fellowship opportunities. This will help recruit scientists that would not normally use genetics or genomic analyses in their studies due to a lack of familiarity and/or facilities. We will also support University curricular activities by sponsoring workshops, laboratory courses and seminars for graduate, undergraduate and K-12 students. NAU has a culturally rich student population with major representation of 42 Native American tribes and Hispanics. We will mentor these students through in-place programs including an NSF sponsored University Mentoring in Environmental Biology (UMEB) grant and a NAU grants program that targets undergraduate research.