Acquisition of a High Capacity Genotyping Facility for Diverse Biological Applications

购买用于多种生物应用的高容量基因分型设备

• 批准号: 0321344
• 负责人: Paul Keim
• 金额: $ 58.18万
• 依托单位: Northern Arizona University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321344&HistoricalAwards=false
• 关键词: Acquisition; Capacity; Genotyping; Facility; Diverse

A grant has been awarded to Northern Arizona University under the direction of Dr. Paul Keim to purchase core instrumentation for a high-capacity, high-resolution DNA genotyping and sequencing facility. The power of genetic and genomic analysis has been well demonstrated in the human and a few selected model genomes, but many other diverse biological species and problems would benefit equally if such technology were readily available. Researchers at Northern Arizona University are involved in diverse genetic and genomic problems that require a state-of-the-art, high capacity facility for DNA analyses. These instruments will be capable of moderate scale DNA sequencing (e.g. a small bacterial genome or single locus across many samples), but more importantly they will provide high capacity genotype analysis through PCR fragment sizing (i.e., DNA fingerprinting). An automated fluorescent detecting DNA sequencer will be used to size PCR fragments associated with microsatellite loci, detect SNPs, perform AFLP, and DNA sequencing. Moderate scale DNA sequencing capacity is valuable for molecular systematics by targeted gene sequencing, or small genome/plasmid sequence determination. The current grant will result in the establishment of a multi-user facility for ENvironmental Genomics and GENetics (EnGGEN). EnGGEN will be a university and regional resource center to provide molecular genetic analyses and training for a diverse user base from regional academic, government and private institutions. Users already committed to the facility are studying plant evolution, ecological community genetics, conservation genetics, microbial diversity, and molecular epidemiology. EnGGEN will provide a complementary facility to the Merriam Powell Center for Environmental Research (MPCER) and the Merriam-Powell Research Station whose missions are to support and foster collaborations among environmental scientists, land-managers, and educators from public and private sectors. Their diverse membership will provide immediate use of our proposed environmental genetics facility and promote its use by a broad research community.To promote the use of this facility throughout the University and regional research community (e.g., Forest Service, National Park Service, TNC, USGS), we have developed a plan of seminars, coursework, hands-on laboratory use, and fellowship opportunities. This will help recruit scientists that would not normally use genetics or genomic analyses in their studies due to a lack of familiarity and/or facilities. We will also support University curricular activities by sponsoring workshops, laboratory courses and seminars for graduate, undergraduate and K-12 students. NAU has a culturally rich student population with major representation of 42 Native American tribes and Hispanics. We will mentor these students through in-place programs including an NSF sponsored University Mentoring in Environmental Biology (UMEB) grant and a NAU grants program that targets undergraduate research.

在Paul Keim博士的指导下，向北方亚利桑那大学提供了一笔赠款，用于为一个高容量、高分辨率的DNA基因分型和测序设施购买核心仪器。遗传和基因组分析的力量已经在人类和一些选定的模型基因组中得到了很好的证明，但如果这种技术随时可用，许多其他不同的生物物种和问题也会同样受益。北方亚利桑那大学的研究人员正在研究各种遗传和基因组问题，这些问题需要最先进的高容量DNA分析设施。这些仪器将能够进行中等规模的DNA测序（例如，小的细菌基因组或跨越许多样品的单个基因座），但更重要的是，它们将通过PCR片段大小测定（即，DNA指纹）。自动荧光检测DNA测序仪将用于确定与微卫星位点相关的PCR片段的大小，检测SNP，进行AFLP和DNA测序。中等规模的DNA测序能力对于通过靶向基因测序或小基因组/质粒序列测定进行分子系统学是有价值的。目前的赠款将导致建立一个多用户设施，用于增强基因组学和遗传学（EngGEN）。EngGEN将成为一所大学和区域资源中心，为来自区域学术、政府和私营机构的不同用户群提供分子遗传分析和培训。已经承诺使用该设施的用户正在研究植物进化、生态群落遗传学、保护遗传学、微生物多样性和分子流行病学。EngGEN将为梅里亚姆鲍威尔环境研究中心（MPCER）和梅里亚姆-鲍威尔研究站提供补充设施，其任务是支持和促进公共和私营部门的环境科学家、土地管理者和教育工作者之间的合作。他们的多元化成员将提供我们拟议的环境遗传学设施的直接使用，并促进其广泛的研究社区的使用。为了促进整个大学和区域研究社区（例如，森林服务，国家公园服务，TNC，美国地质调查局），我们已经制定了一个计划的研讨会，课程，动手实验室的使用，和奖学金的机会。这将有助于招募那些由于缺乏熟悉和/或设施而通常不会在研究中使用遗传学或基因组分析的科学家。我们还将通过赞助研讨会，实验室课程和研讨会，为研究生，本科生和K-12学生支持大学的课程活动。NAU拥有文化丰富的学生群体，主要代表42个美洲原住民部落和西班牙裔。我们将指导这些学生通过就地计划，包括NSF赞助的环境生物学大学辅导（UMEB）补助金和NAU赠款计划，目标是本科生的研究。