Acquisition of DNA Sequencing and Genotyping Instrumentation of Diverse Biological Applications

我们的不同生物学家小组正在积极地使用DNA分析来进行与1)系统学、2)基因组图谱、3)行为生物学、4)濒危物种保护和5)生态遗传学相关的综合研究。我们正在使用DNA序列数据进行系统发育分析，并使用DNA标记来解决种群水平和家庭水平的问题。我们目前从其他大学的远程设施中签约提供DNA测序服务，或者使用放射性标记方法和手动放射自显影评分来生成我们自己的基因数据。我们的建议是为我们的大学带来数据生成的仪器，并增加我们进行这类分析的能力。现场仪器将降低这种分析的成本，增加我们教员对分子分析的使用，提高数据生成的速度，并直接让我们的学生接触正在塑造我们科学的DNA技术的下一代。我们需要这种工具来保持我们的高质量研究项目在国内和国际层面上的竞争力。

Our group of diverse biologists are actively using DNA analysis to conduct integrative research on issues pertaining to 1) systematics, 2) genome mapping, 3) behavioral biology, 4) conservation of endangered species, and 5) ecological genetics. We are using DNA sequence data for phylogenetic analysis and using DNA markers for population-level and family-level questions. We currently contract for DNA sequencing services from distant facilities at other universities or generate our own genotype data using radioactive labeling methods and manual autoradiogram scoring. Our proposal is for instrumentation that will bring data generation to our university and increase our capacity for this type of analysis. On site instrumentation would lower the cost of this analysis, increase the use of molecular analysis by our faculty, increase the speed of data generation, and directly expose our students to the next generation in DNA technology that is shaping our sciences. We need this instrumentation to keep our high quality research programs Competitive at national and international levels.