Development of Diploid Wheat (Triticum Monococcum) Deletion Lines for Reverse Genetics.

小麦是人类饮食中最重要的植物蛋白来源，与其他谷物(大米、玉米、高粱、大麦、小米和燕麦)一起，占人类直接消费的大部分食物。后测序时代面临的挑战是确定测序基因的生物学功能。反向遗传学--通过搜索特定基因中的损伤来发现基因功能--在这一过程中发挥着至关重要的作用。小麦等重要作物物种的庞大而复杂的基因组需要开发大量的突变体，而迄今开发的遗传筛选方法并不支持使这种搜索有效所需的高通量。我们打算优化和演示一种适用于任何大基因组物种的模型筛选系统。研究资源我们将在栽培的二倍体小麦--单粒小麦中产生一系列携带基因组小缺失的品系。通过一种称为处理(检测基因组中的加合物损伤)的方法相结合的汇集方案，可以有效地完成对感兴趣基因中的损伤的突变集合的筛选。我们将通过鉴定影响小麦生殖发育的突变来证明这种方法的实用性。我们还将开发一个数据库WIRE(小麦信息资源)，以传播这次和未来交易实验产生的信息。

Wheat is the single most important source of plant protein in the human diet and together with other cereal grains (rice, corn, sorghum, barley, millet, and oats) accounts for most of the food directly consumed by humans. The challenge for the post-sequencing era is to identify the biological functions of sequenced genes. Reverse genetics - the discovery of gene function by searching for lesions in specific genes - plays an essential role in that process. The large, complex genomes of important crop species like wheat necessitate the development of a large number of mutants, and genetic screening methods developed to date do not support the high throughput necessary for making this search efficient. We intend to optimize and demonstrate a model screening system applicable to any large genome species.Research resourcesWe will produce a collection of lines carrying small deletions in the genome in the cultivated diploid wheat, Triticum monococcum. Screening of this mutant collection for lesions in genes of interest can be accomplished efficiently through a pooling scheme combined with a method termed DEALING (DEtecting Adduct Lesions IN Genomes). We will demonstrate the utility of this approach by identifying mutations that affect reproductive development in wheat. We will also develop a database, WIRE (the Wheat Information Resource), to disseminate the information generated by this and future DEALING experiments.