A Set of Transgenic Maize Lines for Localized Mutagenesis Based on the Ac-Ds Transposon System

一组基于Ac-Ds转座子系统的局部诱变转基因玉米品系

• 批准号: 0321494
• 负责人: Hugo Dooner
• 金额: $ 103.56万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-11-15 至 2007-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321494&HistoricalAwards=false
• 关键词: Set; Transgenic; Maize; Lines; Localized

Maize is the most important American crop today. Therefore, a thorough knowledge of the function of maize genes is highly desirable. That knowledge can be gained from maize functional genomics projects that use transposons to disrupt gene function. In the present maize functional genomics project, a genetic resource is being developed that will enable investigators to combine the power of the mutational approach to define gene function with that of DNA sequencing to define a gene's make-up. The resource consists of a comprehensive set of lines based on transposons of the Ac/Ds family, which because of their transposition pattern, will be useful to scientists interested in saturating specific regions of the maize genome with transposon insertions. A set of 370 randomly generated transgenic lines with active transposon 'launch pads' will be identified, from which we expect to identify 124 transgenic lines that are equally spaced across the genome. In this set most genes in the maize genome will be within 7 cM of a launcing platform and, therefore realistic targets in localized transposon mutagenesis experiments. The transposons have been engineered to greatly facilitate the isolation of DNA sequences adjacent to transposed elements. The simple genetic scheme to select for transpositions and the ease in isolation of the adjacent sequences should make this resource particularly user-friendly. The lines will be made freely available to the research community through the Maize Genetics Stock Center in Urbana, IL. They will be deposited approximately once a year. Broader ImpactsThe ability for researchers to do mutagenesis across the maize genome will enable gene discovery and empower the corn research community.

玉米是当今美国最重要的农作物。因此，深入了解玉米基因的功能是非常必要的。这些知识可以从利用转座子破坏基因功能的玉米功能基因组学项目中获得。在目前的玉米功能基因组学项目中，正在开发一种遗传资源，使研究人员能够将确定基因功能的突变方法与确定基因组成的DNA测序方法结合起来。该资源由一套基于Ac/Ds家族转座子的综合系组成，由于其转座模式，将有助于有兴趣用转座子插入饱和玉米基因组特定区域的科学家。一组370个随机产生的转基因株系与活跃的转座子“发射台”将被确定，我们预计将确定124个转基因株系，在整个基因组中均匀分布。在这一组中，玉米基因组中的大多数基因将在发射平台的7 cM内，因此是定位转座子诱变实验中的现实靶标。转座子已经被工程化以极大地促进邻近转座元件的DNA序列的分离。简单的遗传方案来选择转座和容易隔离的相邻序列应该使这个资源特别用户友好。这些品系将通过伊利诺伊州厄巴纳的玉米遗传储备中心免费提供给研究界。大约每年存入一次。更广泛的影响研究人员在玉米基因组中进行诱变的能力将使基因发现成为可能，并赋予玉米研究界权力。