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Functional role and ecotype divergence in freshwater ultramicrobacteria

淡水超微生物的功能作用和生态型差异

基本信息

批准号：
163678447
负责人：
Professor Dr. Hans-Peter Grossart
金额：
--
依托单位：
Abteilung 3: Plankton- und mikrobielle Ökologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2012-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/163678447?language=en
关键词：
Functional role ecotype divergence freshwater

项目摘要

Prokaryotic microorganisms in freshwater habitats are centrally involved in carbon turnover processes, yet individual microbial populations greatly differ in their respective metabolic and ecological features. The pelagic zone of freshwater systems is typically dominated by freely suspended ultramicrobacteria. The proposed project will study the functional differences and similarities of three monophyletic groups of freshwater ultramicrobacteria (P. necessarius, actinobacteria from the Ac1 clade and alphaproteobacteria affiliated with the LD12 lineage). It will furthermore investigate the sorting of physiologically and genotypically distinct ecotypes within each group by their specific habitats. Single-cell approaches will be combined with (meta)genomic and transcriptomic analyses both on available isolates and on uncultured ultramicrobacteria in experimental studies and comparative in situ investigations in different habitats, and the diversity and expression of functional genes (e.g., bacterial rhodopsins) will be assessed. The different levels of interactions and common activities envisaged in the proposed project will for the first time allow a synoptic view of the functional roles and phylogeographic distribution patterns of freshwater ultramicrobacteria that could not be achieved otherwise. It also offers a chance to European scientists to take a leading role in freshwater microbiology through the concerted development and application of novel functional genomic research tools.

淡水生境中的原生微生物主要参与碳周转过程，但个体微生物种群在各自的代谢和生态特征方面存在很大差异。淡水系统的浮游层通常由自由悬浮的超微细菌控制。拟议的项目将研究淡水超微细菌的三个单系群（P.necessarius、Ac1分支的放线菌和隶属于LD 12谱系的α变形菌）的功能差异和相似性。它还将调查按其特定生境对每组内生理和基因型不同的生态类型进行分类。单细胞方法将与（Meta）基因组和转录组学分析相结合，对实验研究中的可用分离物和未培养的超微细菌进行分析，并在不同生境中进行原位比较研究，以及功能基因的多样性和表达（例如，细菌视紫红质）。拟议项目中设想的不同层次的相互作用和共同活动将首次使人们能够对淡水超微生物菌的功能作用和地理分布模式有一个全面的了解，这是其他方式无法实现的。它还为欧洲科学家提供了一个机会，通过共同开发和应用新的功能基因组研究工具，在淡水微生物学方面发挥主导作用。