Evaluation and quantification of microbial degradation of allochthonous organic matter by "priming" (MicroPrime)
通过“引发”对异地有机物的微生物降解进行评估和定量(MicroPrime)
基本信息
- 批准号:256575941
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2014
- 资助国家:德国
- 起止时间:2013-12-31 至 2018-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The main goal of this project is to characterize future changes in aquatic C-cycling via microbial mechanisms, in particular by focusing on priming effects, which have been little studied in aquatic ecosystems. Depending on environmental conditions, priming can either stimulate or inhibit microbial C-cycling. Therefore, we aim to unravel future changes in the role of priming via compound specific stable isotope analysis (e.g. 13C/12C ratio in microbial phospholipid fatty acids (PLFA)) both in field and laboratory experiments. We intend to developing suitable protocols to combine PLFA analyses with molecular methods, e.g. stable isotope probing (DNA-SIP; RNA-SIP), in particular for bacteria and fungi. This study aims to determine the impact of terrigenous OC input on priming effects, and hence on the aquatic-terrestrial coupling as well as their role for C-cycling in aquatic systems affected by global change. To our knowledge, this will be the first study testing for the metabolic transfer of 13C-labled OC from both, labile and recalcitrant OC-pools in one approach and hence investigating the underlying mechanisms of priming effects in aquatic ecosystems. Both C-pools will be supplied with different pulsing-regimes and nutrient-conditions to unravel effects of labile-OM-availability on recalcitrant organic matter (OM) degradation processes. In the first phase, laboratory experiments consisting of defined microorganisms with well characterized abilities to degrade defined substrates will be carried out and thus enable studies on basic mechanisms, interactions between organisms and their role for OM degradation. Special emphasis will be set on the role of fungi in aquatic mineralization processes. We first want to elucidate basic mechanisms concerning priming, synergistic effects of bacteria and fungi in these processes and quantify which parts of the OM-pool (labile vs. recalcitrant) are respired or incorporated into microbial biomass. In a second phase, the role of characterized basic mechanisms and organism-interactions for OM cycling will be studied using natural communities, different time courses of C-additions (pulsing to simulate more frequent inputs of C in aquatic systems) and different nutrient conditions in the medium (elemental stoichiometry). Later in a third phase, these studies will be extended to much more complex environmental conditions by using mesocosm-experiments (http://www.lake-lab.de/). Here we mainly want to challenge our gained knowledge from phase one and two under environmental conditions, in particular addressing particle fluxes under natural conditions. Our modular approach will enable us to elucidate the role of priming in C-cycling in the water column as well as at aquatic-terrestrial interfaces and hence to improve C-budgets in aquatic systems.
该项目的主要目标是通过微生物机制表征未来水生碳循环的变化,特别是通过关注引发效应,这在水生生态系统中很少研究。根据环境条件,预充可以刺激或抑制微生物C循环。因此,我们的目标是通过化合物特异性稳定同位素分析(例如微生物磷脂脂肪酸(PLFA)中的13 C/12 C比率),在现场和实验室实验中揭示引发作用的未来变化。我们打算开发合适的协议,结合联合收割机PLFA分析与分子方法,如稳定同位素探测(DNA-SIP; RNA-SIP),特别是对细菌和真菌。本研究旨在确定陆源有机碳输入的启动效应的影响,从而对水-陆耦合以及它们的作用,在受全球变化影响的水生系统中的C-循环。据我们所知,这将是第一个研究测试的13 C-标记的OC从两个,不稳定的OC池中的一种方法的代谢转移,从而调查在水生生态系统中的启动效应的潜在机制。这两个C池将提供不同的脉冲制度和营养条件,以解开不稳定的有机质(OM)的降解过程中的有效性的影响。在第一阶段,将进行实验室实验,包括确定的微生物具有良好的表征能力,降解确定的底物,从而使基本机制,生物体之间的相互作用及其作用的OM降解的研究。将特别强调真菌在水生矿化过程中的作用。我们首先要阐明有关启动,在这些过程中的细菌和真菌的协同效应的基本机制,并量化OM池(不稳定与不稳定)的哪些部分被呼吸或纳入微生物生物量。在第二阶段,特征的基本机制和有机体的相互作用OM循环的作用将使用自然社区,不同的时间进程的C-添加(脉冲模拟更频繁的输入C在水生系统)和不同的营养条件下的介质(元素化学计量)进行研究。在第三阶段的后期,这些研究将通过使用中宇宙实验扩展到更为复杂的环境条件(http://www.lake-lab.de/)。在这里,我们主要想挑战我们从第一阶段和第二阶段在环境条件下获得的知识,特别是解决自然条件下的颗粒通量。我们的模块化的方法将使我们能够阐明的作用,启动C-循环中的水柱,以及在水生-陆地界面,从而提高C-预算在水生系统。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Effects of zooplankton carcasses degradation on freshwater bacterial community composition and implications for carbon cycling
浮游动物尸体降解对淡水细菌群落组成的影响及其对碳循环的影响
- DOI:10.1111/1462-2920.14418
- 发表时间:2019
- 期刊:
- 影响因子:0
- 作者:Kolmakova;Gladyshev;Fonvielle;Ganzert;Hornick;Grossart
- 通讯作者:Grossart
Transformation of humic substances by the freshwater Ascomycete Cladosporium sp.
- DOI:10.1002/lno.10545
- 发表时间:2017-09
- 期刊:
- 影响因子:4.5
- 作者:Keilor Rojas‐Jimenez;Jérémy A. Fonvielle;Hua Ma;H. Grossart
- 通讯作者:Keilor Rojas‐Jimenez;Jérémy A. Fonvielle;Hua Ma;H. Grossart
The contrasting roles of aquatic fungi and oomycetes in the degradation and transformation of polymeric organic matter
- DOI:10.1002/lno.11242
- 发表时间:2019-07-16
- 期刊:
- 影响因子:4.5
- 作者:Masigol, Hossein;Khodaparast, Seyed Akbar;Grossart, Hans-Peter
- 通讯作者:Grossart, Hans-Peter
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Professor Dr. Hans-Peter Grossart其他文献
Professor Dr. Hans-Peter Grossart的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Professor Dr. Hans-Peter Grossart', 18)}}的其他基金
Ecological role of fungal parasites on benthic diatoms of polar coastal waters
极地沿海水域底栖硅藻真菌寄生虫的生态作用
- 批准号:
424170377 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Infrastructure Priority Programmes
Alternative states of a simple predator-prey system induced by competition between small edible and large inedible algae and fungal parasitism (APPS)
由小型可食用藻类和大型不可食用藻类之间的竞争以及真菌寄生引起的简单捕食者-猎物系统的替代状态(APPS)
- 批准号:
394716440 - 财政年份:2017
- 资助金额:
-- - 项目类别:
Priority Programmes
Mortality of zooplankton in lake ecosystems and its contribution to vertical carbon fluxes (ZooFlux)
湖泊生态系统中浮游动物的死亡率及其对垂直碳通量的贡献(ZooFlux)
- 批准号:
319532628 - 财政年份:2016
- 资助金额:
-- - 项目类别:
Research Grants
Comparing phenotypic plasticity in bacterial prey traits and ecological consequences by using specialist vs. generalist strains and organic aggregates as model systems
通过使用专业菌株和通才菌株和有机聚集体作为模型系统,比较细菌猎物特征的表型可塑性和生态后果
- 批准号:
257346203 - 财政年份:2014
- 资助金额:
-- - 项目类别:
Priority Programmes
Revising the methane cycling in lakes: sources and sinks in two German lakes with specific consideration of methane accumulation in oxic waters
修改湖泊中的甲烷循环:德国两个湖泊的源和汇,特别考虑含氧水域中的甲烷积累
- 批准号:
241479293 - 财政年份:2013
- 资助金额:
-- - 项目类别:
Research Grants
Bacteria-zooplankton interactions: a key to understanding bacterial dynamics and biogeochemical processes in lakes?
细菌-浮游动物相互作用:了解湖泊细菌动力学和生物地球化学过程的关键?
- 批准号:
201319348 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Research Grants
Functional role and ecotype divergence in freshwater ultramicrobacteria
淡水超微生物的功能作用和生态型差异
- 批准号:
163678447 - 财政年份:2010
- 资助金额:
-- - 项目类别:
Research Grants
Auswirkung interner Wellen auf mikrobielle Habitate kund Aktivitäten sowie den Stoffaustausch an der Wasser-Sediment-Grenzschicht des Stechlinsees
内波对施特奇林湖水-沉积物界面微生物栖息地、活动及物质交换的影响
- 批准号:
75711677 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Research Grants
Veränderungen in der Struktur und Funktion pelagischer Bakterien während und nach der Restauration des Tiefwarensees
蒂夫瓦伦湖恢复期间和恢复后中上层细菌结构和功能的变化
- 批准号:
39400205 - 财政年份:2007
- 资助金额:
-- - 项目类别:
Research Grants
Effects of climate variability on interactions between cyanobacteria and associated microheterotrophs-consequences for development of toxic cyanobacterial blooms
气候变化对蓝藻和相关微型异养生物之间相互作用的影响——对有毒蓝藻水华发展的影响
- 批准号:
28750711 - 财政年份:2006
- 资助金额:
-- - 项目类别:
Priority Programmes
相似国自然基金
Identification and quantification of primary phytoplankton functional types in the global oceans from hyperspectral ocean color remote sensing
- 批准号:
- 批准年份:2022
- 资助金额:160 万元
- 项目类别:
高维半参数模型的稳健统计推断
- 批准号:n/a
- 批准年份:2022
- 资助金额:10.0 万元
- 项目类别:省市级项目
玉米幼苗干旱胁迫应答NAC转录因子基因的筛选和鉴定
- 批准号:31201268
- 批准年份:2012
- 资助金额:25.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Quantification of microbial inactivity rates by high-velocity impacts to improve the reliability of planetary protection for space exploration
通过高速撞击量化微生物不活动率,以提高太空探索行星保护的可靠性
- 批准号:
22K18861 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Performance Quantification/Enhancement Study: Microbial Colouration & Finishing for Textiles?
性能量化/增强研究:微生物着色
- 批准号:
10045702 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Grant for R&D
Quantification of bacterial strain count in the human gut microbiome in health and disease
健康和疾病中人体肠道微生物组中细菌菌株计数的量化
- 批准号:
10387448 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Quantification of bacterial strain count in the human gut microbiome in health and disease
健康和疾病中人体肠道微生物组中细菌菌株计数的量化
- 批准号:
10546433 - 财政年份:2021
- 资助金额:
-- - 项目类别:
Advancing Carbonyl Sulfide (OCS) as an Independent Atmospheric Ttracer for Global Photosynthesis through Quantification of Microbial-mediated Sources and Sinks in Soils
通过量化土壤中微生物介导的源和汇,推进硫化碳 (OCS) 作为全球光合作用的独立大气示踪剂
- 批准号:
1933280 - 财政年份:2020
- 资助金额:
-- - 项目类别:
Standard Grant
A Computational Framework for Protein Identification and Quantification in Metaproteomics Using Data-Independent Acquisition
使用数据独立采集进行元蛋白质组学蛋白质鉴定和定量的计算框架
- 批准号:
10047086 - 财政年份:2020
- 资助金额:
-- - 项目类别:
A Miniaturized Tool for Ultrasound Quantification of Periodontal Disease
牙周病超声定量的小型化工具
- 批准号:
9807257 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Can roots gain the competitive advantage over the soil microbial community? Quantification of spatial niches in Canadian peatlands.
根部能否获得相对于土壤微生物群落的竞争优势?
- 批准号:
533094-2019 - 财政年份:2019
- 资助金额:
-- - 项目类别:
Postdoctoral Fellowships
Development of a novel simple and quick rRNA quantification method for analyzing environmental microbial community structure
开发一种新的简单快速的rRNA定量方法来分析环境微生物群落结构
- 批准号:
17K18896 - 财政年份:2017
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Rapid Identification and Quantification of Organisms from Heterogeneous DNA
异质 DNA 中生物体的快速鉴定和定量
- 批准号:
8885498 - 财政年份:2014
- 资助金额:
-- - 项目类别: