Finishing the Rice Genome

完成水稻基因组

• 批准号: 0321683
• 负责人: William McCombie
• 金额: $ 420.28万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321683&HistoricalAwards=false
• 关键词: Finishing; Rice; Genome

Rice is the most important food source worldwide. The rice genome also serves as a model for the closely related crops such as corn and wheat. The corn and wheat genomes are much larger than that of rice so the rice genome provides a cost effective way to understand these other plants. The International Rice Genome Sequencing Project (IRGSP) completed a high quality draft sequence of the rice genome (Oryza sativa ssp. japonica, cv. Nipponbare) in December 2002 and is now committed to finishing the sequenceThis project continues previous, successful work towards finishing the public rice genome sequence. The 300-350 Bacterial Artificial Chromosome (BAC) clones to be sequenced to finished standards (an error rate of no more than 1/10,000 bases) comprise about 10% of the genome. Support from this award will also allow the project to collaborate with groups from other countries to sequence 1% - 2% of the most technically challenging regions of the genome that remain to be completed. The project will host and train scientists from other countries participating in the IRGSP. Training opportunities will also be available for local high school students and faculty from minority serving institutions in a collaboration between the Cold Spring Harbor Genome Center and the Dolan DNA Learning Center. DeliverablesAll sequence information will be released according to the Bermuda Agreement/Fort Lauderdale principles (http://www.genome.gov/page.cfm?pageID=10506537). All contiguous stretches of sequence (contigs) over 1,500 base pairs in length will be immediately and automatically released at http://nucleus.cshl.edu/riceweb/, including sequence and chromotogram files. Assemblies of BAC sequences will be updated weekly and released to GenBank. The BAC clones sequenced are available through the Clemson University BAC/EST Resource Center (http://www.genome.clemson.edu).

水稻是世界上最重要的食物来源。 水稻基因组也是玉米和小麦等密切相关作物的模型。 玉米和小麦的基因组比水稻大得多，因此水稻基因组提供了一种了解这些其他植物的成本效益高的方法。 国际水稻基因组测序计划（IRGSP）完成了水稻基因组（Oryza sativa ssp.日本Nipponbare）于2002年12月启动，目前致力于完成该序列。该项目延续了之前成功完成公共水稻基因组序列的工作。 待测序至成品标准（错误率不超过1/10，000个碱基）的300-350个细菌人工染色体（BAC）克隆约占基因组的10%。该奖项的支持还将使该项目能够与其他国家的团队合作，对1% - 2%的技术上最具挑战性的基因组区域进行测序。该项目将接待和培训来自参加IRGSP的其他国家的科学家。 在冷泉港基因组中心和多兰DNA学习中心的合作下，当地高中学生和少数民族服务机构的教师也将获得培训机会。 所有序列信息将根据百慕大协议/劳德代尔堡原则（http：//www.example.com）发布www.genome.gov/page.cfm? pageID=10506537）。 长度超过1,500个碱基对的所有连续序列（重叠群）将立即自动发布在http：//nucleus.cshl.edu/riceweb/上，包括序列和色谱图文件。 BAC序列的组装将每周更新并发布到GenBank。 测序的BAC克隆可通过克莱姆森大学BAC/EST资源中心（http：//www.example.com）获得。www.genome.clemson.edu