Meeting on the Future of Plant Genome Sequencing and Analysis to be held May 18-20, 2011 at Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

关于植物基因组测序和分析的未来的会议将于 2011 年 5 月 18 日至 20 日在纽约冷泉港冷泉港实验室举行。

• 批准号: 1135736
• 负责人: William McCombie
• 金额: $ 4.88万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1135736&HistoricalAwards=false
• 关键词: Meeting; Future; Plant; Genome; Sequencing

The last few years have seen a revolution in DNA sequencing instrumentation and technology. In that short time period sequencing instrument capabilities have increased more than 1000 fold and are likely to continue to increase about 5-fold each year for the next several years. As such it is now affordable and efficient to sequence to high coverage large plant genomes that had previously been prohibitively too expensive and complex to attempt. However, analysis methods have not improved nearly as much during the same time period and a variety of technical limitations of these new DNA sequencing instruments make it even more difficult to carry out whole genome sequencing of novel genomes (de novo sequencing). These limitations also make it more difficult to use the new instruments to carry out older clone based strategies for de novo sequencing, such as BAC-by-BAC approaches. The purpose of this meeting to be held at Cold Spring Harbor Laboratory May 18-20, 2011 are to assess the current state of next generation sequencing in terms of de novo, whole genome plant sequencing, what can be expected to develop in the near future, and then determine which advances are needed to allow these exciting technologies to be used to carry out de novo sequencing of entire complex plant genomes. The meeting will bring together stakeholders with broad range of expertise in high-throughput sequencing and genomics, plant biology, bioinformatics and databases drawn from the academic, private and international sectors. Meeting outcomes will be captured in the form of a report to be developed by participants that will be submitted for publication to Genome Research.

过去几年见证了DNA测序仪器和技术的革命。在这短时间内，测序仪器的能力增加了1000多倍，并可能在今后几年每年继续增加约5倍。因此，现在对高覆盖率的大型植物基因组进行测序是负担得起的和有效的，而以前这种测序过于昂贵和复杂而无法尝试。然而，在同一时期，分析方法并没有得到很大的改进，而且这些新的DNA测序仪器的各种技术限制使得对新基因组进行全基因组测序（de novo sequencing）变得更加困难。这些限制也使得使用新仪器来执行旧的基于克隆的从头测序策略更加困难，例如BAC-by-BAC方法。本次会议将于2011年5月18日至20日在冷泉港实验室举行，目的是评估新一代测序的现状，全基因组植物测序，在不久的将来可以预期的发展，然后确定哪些进步需要让这些令人兴奋的技术被用来进行整个复杂植物基因组的新测序。会议将汇集来自学术、私营和国际部门的在高通量测序和基因组学、植物生物学、生物信息学和数据库方面具有广泛专业知识的利益攸关方。会议成果将以报告的形式记录下来，由与会者编写，并提交给《基因组研究》杂志发表。