Meeting on the Future of Plant Genome Sequencing and Analysis to be held May 18-20, 2011 at Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

关于植物基因组测序和分析的未来的会议将于 2011 年 5 月 18 日至 20 日在纽约冷泉港冷泉港实验室举行。

• 批准号: 1135736
• 负责人: William McCombie
• 金额: $ 4.88万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1135736&HistoricalAwards=false
• 关键词: Meeting; Future; Plant; Genome; Sequencing

The last few years have seen a revolution in DNA sequencing instrumentation and technology. In that short time period sequencing instrument capabilities have increased more than 1000 fold and are likely to continue to increase about 5-fold each year for the next several years. As such it is now affordable and efficient to sequence to high coverage large plant genomes that had previously been prohibitively too expensive and complex to attempt. However, analysis methods have not improved nearly as much during the same time period and a variety of technical limitations of these new DNA sequencing instruments make it even more difficult to carry out whole genome sequencing of novel genomes (de novo sequencing). These limitations also make it more difficult to use the new instruments to carry out older clone based strategies for de novo sequencing, such as BAC-by-BAC approaches. The purpose of this meeting to be held at Cold Spring Harbor Laboratory May 18-20, 2011 are to assess the current state of next generation sequencing in terms of de novo, whole genome plant sequencing, what can be expected to develop in the near future, and then determine which advances are needed to allow these exciting technologies to be used to carry out de novo sequencing of entire complex plant genomes. The meeting will bring together stakeholders with broad range of expertise in high-throughput sequencing and genomics, plant biology, bioinformatics and databases drawn from the academic, private and international sectors. Meeting outcomes will be captured in the form of a report to be developed by participants that will be submitted for publication to Genome Research.

过去几年，DNA 测序仪器和技术发生了一场革命。 在这么短的时间内，测序仪器的能力已经提高了 1000 倍以上，并且在接下来的几年里可能每年继续提高约 5 倍。因此，现在可以负担得起且有效地对高覆盖度大型植物基因组进行测序，而以前的尝试过于昂贵和复杂。然而，在同一时期，分析方法并没有得到多大的改进，而且这些新型 DNA 测序仪器的各种技术限制使得对新基因组进行全基因组测序（从头测序）变得更加困难。这些限制也使得使用新仪器执行基于旧克隆的从头测序策略（例如 BAC-by-BAC 方法）变得更加困难。这次会议将于 2011 年 5 月 18 日至 20 日在冷泉港实验室举行，其目的是评估下一代测序在从头、全基因组植物测序方面的现状，以及在不久的将来的预期发展，然后确定需要哪些进展才能使这些令人兴奋的技术用于对整个复杂植物基因组进行从头测序。 会议将汇集来自学术、私营和国际部门的在高通量测序和基因组学、植物生物学、生物信息学和数据库方面拥有广泛专业知识的利益相关者。会议成果将以报告的形式记录下来，由参与者编写，并提交给基因组研究机构发表。