Meeting on the Future of Plant Genome Sequencing and Analysis to be held May 18-20, 2011 at Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

关于植物基因组测序和分析的未来的会议将于 2011 年 5 月 18 日至 20 日在纽约冷泉港冷泉港实验室举行。

• 批准号: 1135736
• 负责人: William McCombie
• 金额: $ 4.88万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1135736&HistoricalAwards=false
• 关键词: Meeting; Future; Plant; Genome; Sequencing

The last few years have seen a revolution in DNA sequencing instrumentation and technology. In that short time period sequencing instrument capabilities have increased more than 1000 fold and are likely to continue to increase about 5-fold each year for the next several years. As such it is now affordable and efficient to sequence to high coverage large plant genomes that had previously been prohibitively too expensive and complex to attempt. However, analysis methods have not improved nearly as much during the same time period and a variety of technical limitations of these new DNA sequencing instruments make it even more difficult to carry out whole genome sequencing of novel genomes (de novo sequencing). These limitations also make it more difficult to use the new instruments to carry out older clone based strategies for de novo sequencing, such as BAC-by-BAC approaches. The purpose of this meeting to be held at Cold Spring Harbor Laboratory May 18-20, 2011 are to assess the current state of next generation sequencing in terms of de novo, whole genome plant sequencing, what can be expected to develop in the near future, and then determine which advances are needed to allow these exciting technologies to be used to carry out de novo sequencing of entire complex plant genomes. The meeting will bring together stakeholders with broad range of expertise in high-throughput sequencing and genomics, plant biology, bioinformatics and databases drawn from the academic, private and international sectors. Meeting outcomes will be captured in the form of a report to be developed by participants that will be submitted for publication to Genome Research.

在过去的几年里，DNA测序仪器和技术发生了一场革命。在这短短的一段时间内，测序仪器的能力增加了1000多倍，并可能在接下来的几年里继续以每年约5倍的速度增长。因此，对以前过于昂贵和复杂得令人望而却步的大型植物基因组进行测序，现在是负担得起的，也是有效的。然而，在同一时期，分析方法几乎没有那么大的进步，这些新的DNA测序仪器的各种技术限制使得对新基因组进行全基因组测序(从头测序)变得更加困难。这些限制也使得使用新仪器来执行旧的基于克隆的从头测序策略变得更加困难，例如BAC-by-BAC方法。本次会议将于2011年5月18日至20日在冷泉港实验室举行，目的是从从头测序的角度评估下一代测序的现状，即全基因组植物测序，以及在不久的将来有望发展什么，然后确定需要哪些进展来允许这些令人兴奋的技术用于对整个复杂植物基因组进行从头测序。这次会议将汇集在高通量测序和基因组学、植物生物学、生物信息学和来自学术界、私营部门和国际部门的数据库方面具有广泛专业知识的利益攸关方。会议成果将以与会者编写的报告的形式记录下来，并提交给基因组研究中心发表。