The role of fungal NADPH oxidase complexes in differentiation and virulence

真菌 NADPH 氧化酶复合物在分化和毒力中的作用

• 批准号: 164177739
• 负责人: Professor Dr. Paul Tudzynski
• 金额: --
• 依托单位: Arbeitsgruppe Molecular Biology and Biotechnology of Fungi (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/164177739?language=en
• 关键词: role; fungal; NADPH; oxidase; complexes

Reactive oxygen species (ROS) are known to play important roles in defense reactions of plants against pathogenic fungi. Research in our lab and elsewhere showed that ROS are also important for developmental processes and virulence for fungi. We study the role of ROS in two plant pathogens with totally different life style: Botrytis cinerea, the grey mould fungus, a necrotroph supporting and probably using the oxidative burst reaction of the host; Claviceps purpurea, the Ergot fungus, a biotrophic pathogen of grasses, avoiding (almost) any defense reaction by mimicking pollen tube growth. We focus on the role of NADPH oxidase complexes, which in both fungi have been shown to be of general importance for differentiation and virulence, though they have very distinct roles in both systems. We will study the specific functions of the two different Nox complexes present in both fungi, their composition, localization, regulation, and their general impact for the maintenance of Redox balance in the fungal cells, with special consideration of the specific adaptations due to the different life styles of the two pathogens, necrotroph vs. biotroph.

活性氧（Reactive oxygen species，ROS）在植物对病原真菌的防御反应中起重要作用。我们实验室和其他地方的研究表明，ROS对真菌的发育过程和毒力也很重要。我们研究了活性氧在两种植物病原体中的作用，这两种植物病原体具有完全不同的生活方式：灰霉病菌，灰霉病菌，一种支持并可能使用宿主的氧化爆发反应的嗜坏死菌;麦角菌，麦角菌，一种禾本科生物营养型病原体，通过模仿花粉管生长来避免（几乎）任何防御反应。我们专注于NADPH氧化酶复合物的作用，这两种真菌已被证明是分化和毒力的一般重要性，虽然他们在这两个系统中有非常不同的作用。我们将研究存在于两种真菌中的两种不同的氮氧化物复合物的具体功能，它们的组成，定位，调节，以及它们对维持真菌细胞中氧化还原平衡的一般影响，特别考虑由于两种病原体的不同生活方式而产生的具体适应性，necrotroph与biotroph。