The role of fungal NADPH oxidase complexes in differentiation and virulence

真菌 NADPH 氧化酶复合物在分化和毒力中的作用

基本信息

项目摘要

Reactive oxygen species (ROS) generated by NADPH oxidases (Nox) play an important role in pathogen defense reactions and a broad variety of differentiation processes in animals and plants. There is growing evidence that also in fungi these membrane complexes are involved in all major developmental processes: they have impact on polar growth, sexual differentiation and virulence. Still there is considerable lack of knowledge on complex composition, recruitment and localization, and on the molecular mechanisms underlying their impact on so many important cellular processes. Based on the broad analysis in the first project period of the Nox complexes and ROS signaling components in general in two fungi with completely different lifestyle and obviously significantly different Nox functions - the biotrophic ergot fungus Claviceps purpurea and the necrotrophic grey mold fungus Botrytis cinerea-, we will focus in this second period on a detailed functional characterization of the Nox complexes in the model system B.cinerea. We will investigate the specific composition/recruitment of the Nox complexes, the localization, linked to specific developmental stages/metabolic activities, focusing on a possible function in the ER. We will study the impact of Nox on the cytoskeleton, in the context of differentiation processes (e.g.penetration structures) and analyse regulatory circuits which affect Nox activity in mammalian systems, e.g. lipid signaling. In addition, we will try to identify alternative ROS sources, based on existing ROS overproducing mutants.
NADPH氧化酶(NOx)产生的活性氧种(ROS)在动植物的病原菌防御反应和多种分化过程中发挥着重要作用。越来越多的证据表明,在真菌中,这些膜复合体也参与了所有主要的发育过程:它们对极地生长、性别分化和毒力有影响。然而,对于复杂的组成、招募和定位,以及它们对许多重要细胞过程的影响的分子机制,仍然缺乏相当大的了解。在第一个项目期对两种生活方式完全不同、NOx功能明显不同的真菌--生物营养麦角菌Clavicep Purpurea和坏死性灰霉菌Botrytis cinerea--的NOx复合体和ROS信号成分进行了广泛的分析基础上,我们将在第二阶段重点研究模式系统B.cinerea中NOx复合体的详细功能特征。我们将研究NOx复合体的特定组成/招募,定位,与特定的发育阶段/代谢活动有关,重点是内质网中可能的功能。我们将在分化过程(例如穿透结构)的背景下研究NOx对细胞骨架的影响,并分析影响哺乳动物系统中NOx活性的调节电路,例如脂质信号。此外,我们将基于现有的ROS高产突变体,尝试寻找替代的ROS来源。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
BcIqg1, a fungal IQGAP homolog, interacts with NADPH oxidase, MAP kinase and calcium signaling proteins and regulates virulence and development in Botrytis cinerea
  • DOI:
    10.1111/mmi.13391
  • 发表时间:
    2016-07-01
  • 期刊:
  • 影响因子:
    3.6
  • 作者:
    Marschall, Robert;Tudzynski, Paul
  • 通讯作者:
    Tudzynski, Paul
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Professor Dr. Paul Tudzynski其他文献

Professor Dr. Paul Tudzynski的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Professor Dr. Paul Tudzynski', 18)}}的其他基金

The role of phytohormone biosynthesis in the Ergot fungus Claviceps purpurea
植物激素生物合成在麦角真菌麦角菌中的作用
  • 批准号:
    256236408
  • 财政年份:
    2014
  • 资助金额:
    --
  • 项目类别:
    Research Grants
The role of fungal NADPH oxidase complexes in differentiation and virulence
真菌 NADPH 氧化酶复合物在分化和毒力中的作用
  • 批准号:
    164177739
  • 财政年份:
    2009
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Early stages of pathogenesis in plant pathogenic fungi
植物病原真菌发病机制的早期阶段
  • 批准号:
    5448854
  • 财政年份:
    2005
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Molecular analysis of ergotalkaloid biosynthesis in Claviceps purpurea
麦角菌麦角生物碱生物合成的分子分析
  • 批准号:
    5405283
  • 财政年份:
    2003
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
"Signalling" bei der Infektion und Kolonisierung von Fruchtknotengewebe von Roggen durch Claviceps purpurea
紫麦角菌感染和定植黑麦卵巢组织中的“信号”
  • 批准号:
    5364503
  • 财政年份:
    2002
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Functional molecular-genetic analysis of the fungal plant pathogen Botrytis cinerea
真菌植物病原体灰葡萄孢的功能分子遗传学分析
  • 批准号:
    5311884
  • 财政年份:
    2001
  • 资助金额:
    --
  • 项目类别:
    Research Grants
The role of reactive oxygen species (ROS) in development and pathogenicity of phytopathogenic fungi
活性氧(ROS)在植物病原真菌发育和致病性中的作用
  • 批准号:
    5189658
  • 财政年份:
    1999
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Die Rolle von Phytohormonen bei der Interaktion zwischen phytopathogenen Pilzen und ihren Wirtspflanzen
植物激素在植物病原真菌与其寄主植物相互作用中的作用
  • 批准号:
    5393236
  • 财政年份:
    1997
  • 资助金额:
    --
  • 项目类别:
    Research Grants

相似海外基金

Renewal application: How do ecological trade-offs drive ectomycorrhizal fungal community assembly? Fine- scale processes with large-scale implications
更新应用:生态权衡如何驱动外生菌根真菌群落组装?
  • 批准号:
    MR/Y011503/1
  • 财政年份:
    2025
  • 资助金额:
    --
  • 项目类别:
    Fellowship
Collaborative Research: The Interplay of Water Condensation and Fungal Growth on Biological Surfaces
合作研究:水凝结与生物表面真菌生长的相互作用
  • 批准号:
    2401507
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
NSF PRFB FY23: Cross-species gene regulation of a plant-fungal symbiosis under environmental stress
NSF PRFB FY23:环境胁迫下植物-真菌共生的跨物种基因调控
  • 批准号:
    2305481
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Fellowship Award
How the mushroom lost its gills: phylogenomics and population genetics of a morphological innovation in the fungal genus Lentinus
蘑菇如何失去鳃:香菇属真菌形态创新的系统基因组学和群体遗传学
  • 批准号:
    2333266
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Tools to Control and Monitor Van der Waals Forces between Nanoparticles: Quantitative Insights on Biological, Environmental, and Fungal Cell Interactions.
控制和监测纳米颗粒之间范德华力的工具:对生物、环境和真菌细胞相互作用的定量见解。
  • 批准号:
    2335597
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Four-stranded fungal fatality: Unveiling G-quadruplexes as future antifungal targets
四链真菌死亡:揭示 G-四链体作为未来抗真菌靶点
  • 批准号:
    BB/Y005058/1
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Research Grant
Study into the effect of candidalysin exotoxin secretion and fungal ROS generation on C. albicans infection on human hepatocytes
念珠菌素外毒素分泌和真菌ROS生成对白色念珠菌感染人肝细胞影响的研究
  • 批准号:
    24K18435
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
NSF Postdoctoral Fellowship in Biology: Priority Effects Within and Between Guilds of Fungal Symbionts
NSF 生物学博士后奖学金:真菌共生体内部和之间的优先效应
  • 批准号:
    2305876
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Fellowship Award
Collaborative Research: The Interplay of Water Condensation and Fungal Growth on Biological Surfaces
合作研究:水凝结与生物表面真菌生长的相互作用
  • 批准号:
    2401506
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
PurSUiT: Biodiversity exploration and phylogenetic systematics of the zoosporic fungal phylum Blastocladiomycota
追求:游动孢子真菌门芽生菌门的生物多样性探索和系统发育系统学
  • 批准号:
    2403677
  • 财政年份:
    2024
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了