Die Rolle von Phytohormonen bei der Interaktion zwischen phytopathogenen Pilzen und ihren Wirtspflanzen

微生物和高等植物的相互作用往往伴随着植物激素水平的变化。双方对这些变化的具体贡献及其对互动的影响尚未解决。在这里，植物激素平衡的作用，致病协会将研究重点灰葡萄孢，休闲剂的几种重要作物的灰霉病，番茄作为其寄主植物。将采取一种综合方法，将整个植物水平的实验与细胞水平的分子和生物化学研究相结合。外源激素对植物对B侵染敏感性的影响将更详细地研究灰霉病（包括植物激素作用或生物合成的抑制剂和混合物）。B产生的植物激素的输入和重要性。植物中的灰霉病将通过生物化学和分子方法相结合进行分析。激素生物合成基因将被克隆和鉴定，重点是脱落酸，赤霉素和乙烯。利用B的生物合成基因。Cinerea为分析植物中真菌途径的表达提供了可能性。转基因非和过量生产菌株将允许特定的真菌植物激素的相互作用的作用的功能分析。B。灰霉病菌系统将与相关的白色真菌核盘菌和活体营养型白粉病真菌Sphaerotheca fusca syn.为了更广泛地了解植物激素的一般作用，在这些研究中将包括具有受阻的激素生物合成和/或改变的对植物激素的敏感性的确定的植物突变体。

Interactions of microorganisms and higher plants often are accompanied by changes in phytohormone levels. The specific contributions of both partners to these changes and their impact for the interaction are not yet resolved. Here the role of phytohormone balance in pathogenic associations will be studied focusing on Botrytis cinerea, the casual agent of grey mould of several important crop plants, and tomato as its host plant. A comprehensive approach will be taken integrating experiments at the whole plant level with molecular and biochemical studies at the cellular level. The influence of external application of phytohormones on the susceptibility of plants to infection by B. cinerea will be investigated in more detail (including inhibitors of phytohormone action or biosyntheses and mixtures). The input and importance of phytohormones produced by B. cinerea in planta will be analysed by combined biochemical and molecular methods. Hormone-biosynthetic gene will be cloned and characterized, focusing on abscisic acid, gibberellins, and ethylene. The availability of biosynthesis genes from B. Cinerea opens the possibility to analyse the expression of the fungal pathways in planta. Transgenic non- and overproducing strains will allow fuctional analyses of the role of specific fungal phytohormones for the interaction. The B. cinerea system will be compared with the related white mould fungus Sclerotinia sclerotiorum and the biotrophic powdery mildew fungus Sphaerotheca fusca syn. fuliginea in order to gain a broader view of the general role of phytohormones. Defined plant mutants with blocked hormone biosynthesis and/or altered susceptibility to plant hormones will bei included in these studies.