Structural and Biochemical Investigations on Fe-Only Hydrogenases
仅铁氢化酶的结构和生化研究
基本信息
- 批准号:0328341
- 负责人:
- 金额:$ 28.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-09-01 至 2006-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The main objective of this projectl is to investigate the structural and catalyticproperties of Fe-Only hydrogenases. Hydrogenases catalyze reversible hydrogenoxidation and function physiologically to either recycle the reduced electron carriers thataccumulate during a fermentative metabolism or couple the oxidation of hydrogen to thegeneration of reduced electron carriers that can be utilized in energy yielding processes.The metal-containing hydrogenases are classified according to their metal content andinclude those that contain both Ni and Fe (NiFe-hydrogenases) and those that contain justFe (Fe-Only hydrogenases). These enzymes are of paramount importance in themetabolism of numerous microorganisms. In addition, since hydrogen is being exploredas a future alternative fuel source, there is considerable interest in the details of thesehydrogen producing biological catalysts. The studies will focus primarily on testing hypotheses thatwere developed as a result of the detailed structural analysis of the Fe-Only hydrogenase(CpI) isolated from the anaerobic soil microorganism Clostridium pasteurianum. The specific objectives are (1) additional structural and biochemical studies on CpI probing a number of remaining key questions with respect to the H Cluster structure and the role of the accessory [FeS] clusters; (2) site-specificamino acid substitution experiments on CpI to examine the role of the polypeptideenvironment of the H Cluster in catalysis and stability; (3) structural characterization ofthe second Fe-Only hydrogenase (CpII) from C. pasteurianum to ascertain its primarysequence and structural relationship to other Fe-Only hydrogenases and to probe thecontributions of the environment of the H Cluster and the accessory FeS clusters indefining hydrogen oxidation versus proton reduction activities in the Fe-Onlyhydrogenases. The above objectives will be pursued by a multidisciplinary approach combiningbiochemical methods, spectroscopic methods and structure determination by X-raydiffraction methods to answer some of the key remaining questions with respect to thestructure and function of Fe-Only hydrogenases.
本课题的主要目的是研究纯铁氢化酶的结构和催化性质。氢化酶催化可逆的氢氧化,生理上的功能是回收在发酵代谢过程中积累的还原电子载体,或将氢的氧化与产生可用于能量产生过程的还原电子载体偶联。含金属氢化酶根据其金属含量进行分类,包括既含Ni又含Fe的氢化酶(NiFe-氢化酶)和仅含Fe(仅含Fe氢化酶)的那些。这些酶在许多微生物的代谢中具有极其重要的作用。此外,由于氢正在被探索作为未来的替代燃料来源,有相当大的兴趣在这些制氢生物催化剂的细节。 这些研究将主要集中在测试的假设,开发作为一个结果的详细结构分析的铁只有氢化酶(CpI)分离自厌氧土壤微生物Clostridium pasteurianum。具体目标是:(1)对CpI进行进一步的结构和生物化学研究,探讨一些关于H簇结构和附属[FeS]簇作用的关键问题:(2)对CpI进行位点特异性氨基酸取代实验,以研究H簇的多肽环境在催化和稳定性中的作用;(3)C. pasteurianum的一级序列和结构关系,并探讨H簇和FeS簇的环境组成对Fe-Onlyhydrogenases的氢氧化和质子还原活性的影响。 上述目标将通过多学科方法结合生物化学方法,光谱方法和X射线衍射方法的结构测定来实现,以回答一些关键的剩余问题,关于铁只有氢化酶的结构和功能。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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John Peters其他文献
Blue-light cystoscopy for multi-disciplinary surveillance of high risk non-muscle invasive bladder cancer
- DOI:
10.1016/j.ijsu.2011.07.202 - 发表时间:
2011-01-01 - 期刊:
- 影响因子:
- 作者:
Mahreen Hussain;Benjamin Lamb;Paula Allchorne;Chi-Ying Li;James Green;John Peters - 通讯作者:
John Peters
Feasibility and Preliminary Effectiveness of an Exercise Program For Rural Survivors of Cancer With Fatigue
- DOI:
10.1016/j.apmr.2022.12.087 - 发表时间:
2023-03-01 - 期刊:
- 影响因子:
- 作者:
Ryan Marker;Andrew Kittelson;Jared Scorsone;Ian Moran;John Peters;Heather Leach - 通讯作者:
Heather Leach
Static energy deserves greater emphasis in the meteorology community
静态能量值得气象界更加重视
- DOI:
10.1175/bams-d-22-0013.1 - 发表时间:
2023 - 期刊:
- 影响因子:8
- 作者:
D. Chavas;John Peters - 通讯作者:
John Peters
Processing titanium aluminide foils
- DOI:
10.1007/bf03220325 - 发表时间:
1989-09-01 - 期刊:
- 影响因子:2.300
- 作者:
Corrado Bassi;John Peters;Jerry Wittenauer - 通讯作者:
Jerry Wittenauer
Enhancing access of combat-wounded veterans to specialist rehabilitation services: the VA Polytrauma Telehealth Network.
增加战斗受伤退伍军人获得专业康复服务的机会:VA Polytrauma Telehealth Network。
- DOI:
10.1016/j.apmr.2007.07.027 - 发表时间:
2008 - 期刊:
- 影响因子:4.3
- 作者:
A. Darkins;Cathy M Cruise;Michael Armstrong;John Peters;Michael Finn - 通讯作者:
Michael Finn
John Peters的其他文献
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{{ truncateString('John Peters', 18)}}的其他基金
Collaborative Research: Understanding Downdrafts in Deep Convection
合作研究:了解深层对流中的下沉气流
- 批准号:
2149353 - 财政年份:2022
- 资助金额:
$ 28.4万 - 项目类别:
Standard Grant
Improving Our Understanding of Pressure Perturbations in Cumulus Convection
提高我们对积云对流压力扰动的理解
- 批准号:
1841674 - 财政年份:2019
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$ 28.4万 - 项目类别:
Interagency Agreement
Connecting Physics and Engineering through a Modernization of the Advanced Laboratory Curriculum (CPE-MALC)
通过现代化的高级实验室课程将物理和工程联系起来(CPE-MALC)
- 批准号:
1712389 - 财政年份:2017
- 资助金额:
$ 28.4万 - 项目类别:
Standard Grant
Engineering Synthetic Symbiosis Between Plant and Bacteria to Deliver Nitrogen to Crops
工程植物和细菌之间的合成共生为作物提供氮
- 批准号:
1753917 - 财政年份:2017
- 资助金额:
$ 28.4万 - 项目类别:
Standard Grant
AGS-PRF: Daytime to Nocturnal Convective Transition in the Central United States
AGS-PRF:美国中部白天到夜间的对流转变
- 批准号:
1524435 - 财政年份:2015
- 资助金额:
$ 28.4万 - 项目类别:
Fellowship Award
Role of the P Clusters and FeMo-Cofactors in Nitrogenase Catalysis
P 簇和 FeMo 辅因子在固氮酶催化中的作用
- 批准号:
1330807 - 财政年份:2013
- 资助金额:
$ 28.4万 - 项目类别:
Standard Grant
Engineering Synthetic Symbiosis Between Plant and Bacteria to Deliver Nitrogen to Crops
工程植物和细菌之间的合成共生为作物提供氮
- 批准号:
1331098 - 财政年份:2013
- 资助金额:
$ 28.4万 - 项目类别:
Standard Grant
The Thermal Biology Institute Research and Education Facility Renovation
热生物学研究所研究和教育设施改造
- 批准号:
0963175 - 财政年份:2010
- 资助金额:
$ 28.4万 - 项目类别:
Standard Grant
16th International Congress on Nitrogen Fixation in Big Sky, Montana
第十六届国际固氮大会在蒙大拿州大天空举行
- 批准号:
0848271 - 财政年份:2009
- 资助金额:
$ 28.4万 - 项目类别:
Standard Grant
Civic Engagement in Non-Majors Introductory Biology: Connecting Problem-Based Learning and Scientific Inquiry
非专业生物学入门的公民参与:将基于问题的学习和科学探究联系起来
- 批准号:
0410720 - 财政年份:2004
- 资助金额:
$ 28.4万 - 项目类别:
Standard Grant
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