Role of the P Clusters and FeMo-Cofactors in Nitrogenase Catalysis
P 簇和 FeMo 辅因子在固氮酶催化中的作用
基本信息
- 批准号:1330807
- 负责人:
- 金额:$ 45.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-09-01 至 2017-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The proposed work involves implementing a multidisciplinary approach using basic biochemistry, spectroscopy, and x-ray crystallography techniques to probe some of the key outstanding issues regarding the mechanism of the key enzyme in nitrogen fixation, nitrogenase. We will probe the hypothesis that the redox-dependent structural changes are key to modulating the midpoint potential and defining the role of the P cluster in gated intermolecular electron transfer. The combined use of key site-directed variants and a europium complex redox mediator will facilitate the capture of defined states of the enzyme relevant to the catalytic mechanism that are not possible to observe in the native state, and will answer questions regarding substrate binding to the FeMo-cofactor. Basic research on biological nitrogen fixation could contribute to less energy demanding and more environmentally sound mechanisms to meet the growing demands for fixed nitrogen associated with global population growth, as the availability of fixed nitrogen sources for plant growth is a limiting factor in global nutrition and the production of nitrogenous fertilizers is very energy demanding and can have negative environmental implications. Broader Impacts include student training and significant contributions to training K-12 teachers through the development of an online course on Agriculture and Energy along with other efforts aimed at teachers in remote rural areas that include tribal schools.
拟议的工作涉及实施一种多学科的方法,使用基础生物化学、光谱学和X射线结晶学技术来探索关于固氮关键酶--固氮酶的一些关键悬而未决的问题。我们将探讨这一假设,即依赖于氧化还原的结构变化是调节中点电位和确定P团簇在门控分子间电子转移中的作用的关键。结合使用关键的定点突变体和Eu络合物氧化还原介体,将有助于捕获与催化机制相关的酶的定义状态,而这些状态在自然状态下是不可能观察到的,并将回答有关底物与FEMO辅因子结合的问题。生物固氮的基础研究有助于减少对能源的需求,建立更无害环境的机制,以满足与全球人口增长相关的对固定氮日益增长的需求,因为可用于植物生长的固定氮源是全球营养的一个限制因素,而氮肥的生产非常耗能,并可能对环境产生负面影响。更广泛的影响包括学生培训和通过开发农业和能源在线课程以及针对包括部落学校在内的偏远农村地区的教师的其他努力,对培训K-12教师作出重大贡献。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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John Peters其他文献
Blue-light cystoscopy for multi-disciplinary surveillance of high risk non-muscle invasive bladder cancer
- DOI:
10.1016/j.ijsu.2011.07.202 - 发表时间:
2011-01-01 - 期刊:
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Mahreen Hussain;Benjamin Lamb;Paula Allchorne;Chi-Ying Li;James Green;John Peters - 通讯作者:
John Peters
Feasibility and Preliminary Effectiveness of an Exercise Program For Rural Survivors of Cancer With Fatigue
- DOI:
10.1016/j.apmr.2022.12.087 - 发表时间:
2023-03-01 - 期刊:
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- 作者:
Ryan Marker;Andrew Kittelson;Jared Scorsone;Ian Moran;John Peters;Heather Leach - 通讯作者:
Heather Leach
Static energy deserves greater emphasis in the meteorology community
静态能量值得气象界更加重视
- DOI:
10.1175/bams-d-22-0013.1 - 发表时间:
2023 - 期刊:
- 影响因子:8
- 作者:
D. Chavas;John Peters - 通讯作者:
John Peters
Processing titanium aluminide foils
- DOI:
10.1007/bf03220325 - 发表时间:
1989-09-01 - 期刊:
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Corrado Bassi;John Peters;Jerry Wittenauer - 通讯作者:
Jerry Wittenauer
Enhancing access of combat-wounded veterans to specialist rehabilitation services: the VA Polytrauma Telehealth Network.
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- DOI:
10.1016/j.apmr.2007.07.027 - 发表时间:
2008 - 期刊:
- 影响因子:4.3
- 作者:
A. Darkins;Cathy M Cruise;Michael Armstrong;John Peters;Michael Finn - 通讯作者:
Michael Finn
John Peters的其他文献
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{{ truncateString('John Peters', 18)}}的其他基金
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2149353 - 财政年份:2022
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$ 45.04万 - 项目类别:
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$ 45.04万 - 项目类别:
Interagency Agreement
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通过现代化的高级实验室课程将物理和工程联系起来(CPE-MALC)
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1753917 - 财政年份:2017
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$ 45.04万 - 项目类别:
Standard Grant
AGS-PRF: Daytime to Nocturnal Convective Transition in the Central United States
AGS-PRF:美国中部白天到夜间的对流转变
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1524435 - 财政年份:2015
- 资助金额:
$ 45.04万 - 项目类别:
Fellowship Award
Engineering Synthetic Symbiosis Between Plant and Bacteria to Deliver Nitrogen to Crops
工程植物和细菌之间的合成共生为作物提供氮
- 批准号:
1331098 - 财政年份:2013
- 资助金额:
$ 45.04万 - 项目类别:
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The Thermal Biology Institute Research and Education Facility Renovation
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0963175 - 财政年份:2010
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16th International Congress on Nitrogen Fixation in Big Sky, Montana
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0848271 - 财政年份:2009
- 资助金额:
$ 45.04万 - 项目类别:
Standard Grant
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0410720 - 财政年份:2004
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$ 45.04万 - 项目类别:
Standard Grant
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- 批准号:
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