SENSORS: Electrochemical Impedance Architecture for Biosensors

传感器：生物传感器的电化学阻抗架构

• 批准号: 0329698
• 负责人: Ian Suni
• 金额: --
• 依托单位: Clarkson University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0329698&HistoricalAwards=false
• 关键词: SENSORS; Electrochemical; Impedance; Architecture; Biosensors

The objective of this project is to develop a new detection scheme for biosensing. This involves both biosensor transduction by electrochemical impedance spectroscopy (EIS) and receptor protein genetic engineering for surface immobilization. EIS can probe both charge transfer and capacitance at the biosensor interface, providing more information than competing methods that involve only amperometric detection. The general applicability of this approach using receptor proteins that undergo large amplitude motion during small-molecule ligand binding will be explored. This EIS detection scheme is coupled to the development of methods for oriented immobilization of genetically engineered proteins onto a gold electrode surface. The ultimate goal of this research is the development of general methods for detection of both small and large ligands and substrates of biological interest. In terms of the broader impacts, the areas of clinical diagnostics, agriculture, national defense, and the drug industries may benefit. The project will provide graduate education and training for students in a highly interdisciplinary field, and the results of this research will be disseminated over the Internet.

本计画的目标是发展一种新的生物感测侦测系统。这涉及电化学阻抗谱（EIS）和受体蛋白基因工程表面固定的生物传感器转导。EIS可以探测生物传感器界面的电荷转移和电容，比仅涉及安培检测的竞争方法提供更多的信息。 将探讨这种方法的普遍适用性，使用受体蛋白，在小分子配体结合过程中经历大幅度的运动。该EIS检测方案与将基因工程蛋白定向固定到金电极表面上的方法的开发相结合。本研究的最终目标是开发用于检测生物感兴趣的小和大配体和底物的通用方法。就更广泛的影响而言，临床诊断、农业、国防和制药工业等领域可能会受益。 该项目将在一个高度跨学科的领域为学生提供研究生教育和培训，研究结果将通过因特网传播。