NER: A Novel Nanobiosensor Architecture

NER：一种新型纳米生物传感器架构

• 批准号: 0304143
• 负责人: Ian Suni
• 金额: $ 10万
• 依托单位: Clarkson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0304143&HistoricalAwards=false
• 关键词: NER; Novel; Nanobiosensor; Architecture

Proposal No: 304143Title: NER: A Novel Nanobiosensor ArchitectureAbstract We propose to develop a new nanobiosensor architecture based on chemical deposition of gold nanowires into a commercially available polymer membrane, followed by chemical mechanical planarization (CMP), a processing step widely employed in the semiconductor industry for producing extremely smooth surfaces. A smooth surface is necessary to allow the use of specific chemistries to attach enzymes and other proteins to the biosensor surface. This surface can be patterned using the tip of an atomic force microscope (AFM), allowing different proteins to be attached in different areas. The ultimate goal is a nanowire array seamlessly embedded into a polycarbonate membrane that can be patterned to allow parallel detection of different molecules.This new nanobiosensor architecture will be validated by immobilizing glucose oxidase onto the gold nanowires. The detection limit of this glucose biosensor will then be determined by measuring the electrochemical current in solutions of varying glucose concentration. In addition, this biosensor will be tested using more advanced electrochemical methods that can also measure the surface capacitance. This nanobiosensor architecture has a number of advantages relative to other electrochemical biosensors. These include the capability for single molecule detection and for miniaturization, biocompatibility, and compatibility with many different detection methods. The impact of this research is potentially quite broad, with the possible development of a new generation of gold/polycarbonate biosensors, including sensors for monitoring living organisms. This could lead to significant advances in the fields of clinical diagnostics, medical implants, environmental monitoring, homeland security, and the food industry. The results of this research will be broadly disseminated, including the Internet, using the PI's multimedia authoring expertise, which was developed through NSF-sponsored curriculum development projects.

提案编号：304143标题：NER：一种新的纳米生物传感器架构摘要我们提出开发一种新的纳米生物传感器架构的基础上化学沉积的金纳米线到市售的聚合物膜，然后通过化学机械平面化（CMP），广泛采用在半导体工业中的处理步骤，用于生产非常光滑的表面。 光滑的表面是必要的，以允许使用特定的化学物质将酶和其他蛋白质附着到生物传感器表面。 这个表面可以使用原子力显微镜（AFM）的尖端进行图案化，允许不同的蛋白质附着在不同的区域。 最终的目标是将纳米线阵列无缝嵌入聚碳酸酯膜中，这种聚碳酸酯膜可以被图案化以允许并行检测不同的分子。这种新的纳米生物传感器架构将通过将葡萄糖氧化酶固定在金纳米线上来验证。 然后通过测量不同葡萄糖浓度溶液中的电化学电流来确定该葡萄糖生物传感器的检测限。 此外，这种生物传感器将使用更先进的电化学方法进行测试，这些方法也可以测量表面电容。 这种纳米生物传感器结构相对于其他电化学生物传感器具有许多优点。 这些包括单分子检测和微型化的能力、生物相容性以及与许多不同检测方法的兼容性。 这项研究的影响可能相当广泛，可能开发新一代的金/聚碳酸酯生物传感器，包括用于监测生物体的传感器。 这可能会导致临床诊断，医疗植入，环境监测，国土安全和食品工业领域的重大进展。 这项研究的结果将广泛传播，包括互联网，利用PI的多媒体创作的专业知识，这是通过国家科学基金会赞助的课程开发项目开发。