ME: Metabolic Engineering of Photosynthetic Bacteria for Enhanced Carbon Fixation and Product Synthesis

ME：用于增强碳固定和产品合成的光合细菌代谢工程

• 批准号: 0331364
• 负责人: Gregory Stephanopoulos
• 金额: $ 51.44万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-11-01 至 2007-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0331364&HistoricalAwards=false
• 关键词: ME; Metabolic; Engineering; Photosynthetic; Bacteria

0331364StephanopoulosThe general objective of this research program is to understand the regulation and flux control of central carbon metabolism in photosynthetic microorganisms and in particular the interaction of central carbon metabolic reactions and downstream pathways of carbon utilization for product synthesis. The specific aims of the present research are to: (i) evaluate the effect of modifications in specific single-gene expression on the rate of growth, CO2 fixation and biopolymer product synthesis, (ii) investigate the potential of modifications in multiple-gene expression for achieving improved phenotypes, (iii) elucidate the mechanism of regulation of the biopolymer synthesis pathway to allow high production under realistic levels of CO2 partial pressures, and (iv) evaluate the physiology of promising recombinant Synechocystis strains.

0331364 Stephanopoulos本研究计划的总体目标是了解光合微生物中中心碳代谢的调节和通量控制，特别是中心碳代谢反应和产物合成碳利用下游途径的相互作用。 本研究的具体目标是：（i）评估特定单基因表达中的修饰对生长速率、CO2固定和生物聚合物产物合成的影响，（ii）研究多基因表达中的修饰用于实现改善的表型的潜力，（iii）阐明生物聚合物合成途径的调节机制，以允许在现实水平的CO2分压下的高产量，和（iv）评价有前景的重组集胞藻属菌株的生理学。