Metabolic Engineering of Carbon Fixation and Utilization for Biopolymer Production by Cyanobacteria (TSE99-B)

蓝藻生物聚合物生产中碳固定和利用的代谢工程（TSE99-B）

• 批准号: 9985421
• 负责人: Gregory Stephanopoulos
• 金额: $ 54.26万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9985421&HistoricalAwards=false
• 关键词: Metabolic; Engineering; Carbon; Fixation; Utilization

Cyanobacter have evolved efficient metabolic pathways for the fixation of carbon dioxide (CO2) and its conversion to various metabolic products. This capability can be exploited in the development of processes for biological CO2 sequestration in combination with the production of useful products. Polyhydroxyalkonates (PHA) represent such a group of product compounds whose biosynthesis, in the case of photoautotrophic organisms, derives from the CO2 incorporated into the cells through photosynthesis. The goals of this project are to: (1) establish transformed strains of cyanobacteria with increased PHA production levels as well as improved PHA compositions; and (2) carry out basic kinetic studies of CO2 fixation and PHA formation in batch as well as continuous culture systems for wild type and transformed cyanobacter strains.

蓝藻已经进化出有效的代谢途径，用于固定二氧化碳（CO2）并将其转化为各种代谢产物。 这种能力可用于开发生物CO2封存工艺，并结合有用产品的生产。 聚羟基烷酸酯（PHA）代表这样一组产物化合物，在光合自养生物的情况下，其生物合成来源于通过光合作用掺入细胞中的CO2。 本项目的目标是：（1）建立具有增加的PHA生产水平以及改进的PHA组成的蓝藻转化菌株;和（2）对野生型和转化蓝藻菌株进行分批以及连续培养系统中的CO2固定和PHA形成的基础动力学研究。