Application of Metabolic Engineering to Amino Acid Overproduction

9311509 Stephanopoulos该项目是关于谷氨酸棒杆菌代谢途径的修饰研究，用于生产天冬氨酸家族的氨基酸（赖氨酸和苏氨酸）。 具体而言，在磷酸烯醇-丙酮酸（PEP），丙酮酸（PYR），和乙酰半醛（阿萨）交界处的流量将被重新分配，以提高产品产量，在氨基酸生产的成本结构中最关键的组成部分。 这将通过一个计划来实现，该计划将尝试在PEP、PYR和阿萨交界处进行酶的去调节、扩增和同步化，此外还进行发酵研究，该研究将在最佳生物反应器系统的设计中利用改善的细胞特性。 ***

9311509 Stephanopoulos This project is on research for modification of the metabolic pathway in Corynebacterium glutamicum for the production of amino acids of the aspartic acid family (lysine and threonine). Specifically, the fluxes at the phosphoenol-pyruvate (PEP), pyruvate (PYR), and aspartyl-semialdehyde (ASA) junctions are to be redistributed to improve product yields, the most critical component in the cost structure of amino acid manufacturing. This will be accomplished by a program that will attempt enzyme deregulation, amplification and synchronization at the PEP, PYR and ASA junctions, in addition to fermentation studies that will exploit the improved cellular properties in the design of an optimal bioreactor system. ***