Application of Metabolic Engineering to Amino Acid Overproduction

本项目是对谷氨酸棒状杆菌代谢途径进行修饰，以生产天冬氨酸家族氨基酸（赖氨酸和苏氨酸）的研究。具体来说，磷酸烯醇-丙酮酸（PEP）、丙酮酸（PYR）和天冬氨酸-半醛（ASA）连接处的通量将被重新分配，以提高产品收率，这是氨基酸制造成本结构中最关键的组成部分。这将通过一个项目来完成，该项目将尝试在PEP、PYR和ASA连接处放松酶的管制、扩增和同步，此外还将进行发酵研究，利用改进的细胞特性设计最佳生物反应器系统。＊＊＊

9311509 Stephanopoulos This project is on research for modification of the metabolic pathway in Corynebacterium glutamicum for the production of amino acids of the aspartic acid family (lysine and threonine). Specifically, the fluxes at the phosphoenol-pyruvate (PEP), pyruvate (PYR), and aspartyl-semialdehyde (ASA) junctions are to be redistributed to improve product yields, the most critical component in the cost structure of amino acid manufacturing. This will be accomplished by a program that will attempt enzyme deregulation, amplification and synchronization at the PEP, PYR and ASA junctions, in addition to fermentation studies that will exploit the improved cellular properties in the design of an optimal bioreactor system. ***