ME: Microbial Synthesis of 1,2,4-Butanetriol

ME：1,2,4-丁三醇的微生物合成

• 批准号: 0331374
• 负责人: John Frost
• 金额: $ 20万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0331374&HistoricalAwards=false
• 关键词: ME; Microbial; Synthesis; Butanetriol

0331374FrostIn this project, biosynthetic pathways are to be created for the synthesis of 1,2,4-butanetriol. Biosynthetic pathways are to be designed and constructed for the synthesis of D-1,2,4-butanetriol from D-xylose and the synthesis of L-1,2,4-butanetriol from L-arabinose. Biosynthetic pathways will also be designed and constructed for the synthesis of D-1,2,4-butanetriol, L-1,2,4-butanetriol and mixtures of these enantiomers from erythritol. D-Xylose and L-arabinose can be derived from abundantly available corn fiber. Erythritol is a fermentation product produced from D-glucose, which can be derived from abundantly available corn starch. Each biosynthetic pathway to be created will be expressed in a separate microbial construct. Fermentor-controlled cultivation of the microbial constructs with pentose or erythritol will lead to synthesis of the desired 1,2,4-butantriol enantiomer(s).

0331374霜冻在这个项目中，生物合成途径将被创建为1，2，4-丁三醇的合成。设计并构建了由D-木糖合成D-1，2，4-丁三醇和由L-阿拉伯糖合成L-1，2，4-丁三醇的生物合成途径。 还将设计和构建生物合成途径，用于从β-D-1，2，4-丁三醇、L-1，2，4-丁三醇以及这些对映体的混合物的合成。D-木糖和L-阿拉伯糖可以从丰富的玉米纤维中获得。 赤藓糖醇是由D-葡萄糖产生的发酵产物，其可以从大量可用的玉米淀粉中获得。要创建的每条生物合成途径将在单独的微生物构建体中表达。用戊糖或麦芽糖在发酵罐中控制培养微生物构建体将导致合成所需的1，2，4-丁三醇对映体。