Application of Different Methodologies to Identify Sources of Fecal Pollution in The Rio Grande River

应用不同方法识别格兰德河粪便污染源

• 批准号: 0331908
• 负责人: Maria Alvarez
• 金额: $ 5万
• 依托单位: El Paso County Community College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0331908&HistoricalAwards=false
• 关键词: Application; Different; Methodologies; Identify; Sources

The U.S.-Mexico Border region has unique problems related to water resources. The Rio Grande/Rio Bravo is the major watershed of the bi-national region. The river serves as an important natural resource for industry, agriculture, domestic water supply, recreation, and wildlife habitat fro both countries. Unfortunately, the Rio Grande is also a conduit for infectious microorganisms and toxic pollutants. A variety of activities contributing to the chemical and microbial contamination of source water have been identified and include improperly installed and maintained septic systems, landfill, injection wells, land application of waste, irrigation, runoff, and animal feed lots. This project is a collaborative effort between El Paso County Community College and the Water Quality Center at Arizona State University. The objective of this project is to conduct a comprehensive and comparative study to determine the source of fecal contamination at four sites of the Rio Grande river using 1) Antibiotic Resistance Analysis of fecal streptococci, 2) Antibiotic Resistance Analysis of E coli, 3) Ribotyping of selected E. coli isolates, and 4) Genotyping of E. coli and F-RNA bacteriophages.

美国-墨西哥边境地区存在与水资源相关的独特问题。 格兰德河/布拉沃河是两国地区的主要分水岭。 这条河是两国工业、农业、生活供水、娱乐和野生动物栖息地的重要自然资源。 不幸的是，格兰德河也是传染性微生物和有毒污染物的传播渠道。 已查明造成水源水化学和微生物污染的各种活动，包括化粪池系统安装和维护不当、垃圾填埋场、注入井、废物土地利用、灌溉、径流和动物饲料场。 该项目是埃尔帕索县社区学院和亚利桑那州立大学水质中心之间的合作项目。 该项目的目标是进行一项全面的比较研究，以确定格兰德河四个地点的粪便污染源，使用 1) 粪便链球菌的抗生素耐药性分析，2) 大肠杆菌的抗生素耐药性分析，3) 选定的大肠杆菌分离株的核糖分型，以及 4) 大肠杆菌和 F-RNA 噬菌体的基因分型。