YIA-PGR: Proteomics of Seed-Filling in Oilseeds

YIA-PGR：油籽种子填充的蛋白质组学

• 批准号: 0332418
• 负责人: Jay Thelen
• 金额: --
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2010-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0332418&HistoricalAwards=false
• 关键词: YIA; PGR; Proteomics; Seed; Filling

Plant seeds are important, renewable sources of natural products such as oil, protein, starch and fiber. Though the biosynthetic pathways for these storage compounds are mostly known it is not clear how these pathways are regulated in oilseeds which produce higher quantities of oil and protein. Quantitation and identification of proteins expressed at multiple stages during embryo development of four diverse oilseeds will systematically reveal the dynamics of protein networks responsible for oil and protein accumulation in four diverse oilseeds. Public dissemination of these data in multiple, user-intuitive formats will provide new directions for the scientific community to engineer seed metabolism for the benefit of health and environment. The website will be open for viewing within the first year of the proposal and will include both one- and two-dimensional gels of membrane and total proteins, respectively, from various stages of oilseed development (within first two-three years of the proposal). Identification of proteins will commence with the purchase of a tandem mass spectrometer in the third year of funding. Identities of the first 300 protein spots (project goal of 500-600) of Arabidopsis and oilseed rape will be posted to the website by the end of the third year support and prior to publication. Identification of 450-500 castor and soybean proteins will also commence in the third year of funding with a projected release date to begin in year four. Manual de novo sequencing of unidentified proteins and potential post-translationally modified proteins will be the subject of research for year five.

植物种子是油、蛋白质、淀粉和纤维等天然产物的重要可再生来源。虽然这些储存化合物的生物合成途径大多是已知的，但尚不清楚这些途径如何在产生更高量的油和蛋白质的油籽中调节。 定量和鉴定的蛋白质在胚胎发育过程中的四个不同的油料种子的多个阶段的表达将系统地揭示蛋白质网络的动态负责油和蛋白质的积累在四个不同的油料种子。 以多种用户直观的格式公开传播这些数据将为科学界提供新的方向，以工程种子代谢，造福健康和环境。 该网站将在提案的第一年内开放供浏览，并将分别包括来自油料种子开发各个阶段的膜和总蛋白质的一维和二维凝胶（提案的前两三年内）。 蛋白质的鉴定将在第三年开始购买串联质谱仪。 拟南芥和油菜的前300个蛋白质点（项目目标为500-600个）的身份将在第三年支持结束前和出版前发布到网站上。 450-500种蓖麻和大豆蛋白的鉴定也将在资助的第三年开始，预计发布日期将在第四年开始。 未识别蛋白质和潜在的后修饰蛋白质的手动从头测序将是第五年的研究主题。