Identification and Absolute Quantitation of Protein Phosphorylation Networks in Oilseeds

油料种子中蛋白质磷酸化网络的鉴定和绝对定量SPI：Jay J. Thelen，Department of Biochemistry，University of Missouri-Columbia合作者：Dong Xu，Computer Science Department，University of密苏里州哥伦比亚植物种子是重要的可再生天然产物来源，如油、蛋白质、淀粉和纤维。 虽然这些储存化合物的生物合成途径大多是已知的，但尚不清楚这些途径如何在产生更高量的油和蛋白质的油籽中调节。 蛋白质磷酸化是许多代谢酶的重要调节机制，初步数据揭示了油菜和大豆发育中的数百种磷蛋白。 鉴定和定量磷蛋白控制机制，在发展中国家的三种不同的油料种子将提供基础，了解参与油料种子开发的监管网络，并将有助于设计新的策略，作物改良。 该提案旨在解开网络的磷蛋白和蛋白激酶主要参与调节过程中的油和蛋白质积累阶段的种子发育在三种不同的油料作物，油菜，大豆和拟南芥的代谢过程。 除了这一基本科学目标外，该提案的更广泛影响包括：（1）组织蛋白质组学主题研讨会，并分别针对高中教师和学生开发基于网络的蛋白质组学教程;（2）在新兴的蛋白质组学学科中培养本科到博士后水平的植物科学家;（3）通过大学资助的本科研究项目和奖学金招募代表性不足的学生;（4）利用新兴的绝对定量技术（AQUA）开发磷酸肽鉴定和定量技术这些研究包括：（1）为植物蛋白磷酸化群体提供合成AQUA肽的工具;（2）为植物蛋白磷酸化群体提供合成AQUA肽的工具;（3）以多种用户直观的格式公开传播磷酸化蛋白质组学数据，为科学界提供新的方向，以修改种子代谢，造福健康和环境。 网站见http://oilseedproteomics.missouri.edu

IDENTIFICATION AND ABSOLUTE QUANTITATION OF PROTEIN PHOSPHORYLATION NETWORKS IN OILSEEDSPI: Jay J. Thelen, Department of Biochemistry, University of Missouri-ColumbiaCollaborator: Dong Xu, Computer Science Department, University of Missouri ColumbiaPlant seeds are important, renewable sources of natural products such as oil, protein, starch and fiber. Though the biosynthetic pathways for these storage compounds are mostly known, it is not clear how these pathways are regulated in oilseeds which produce higher quantities of oil and protein. Protein phosphorylation is an important regulatory mechanism for many metabolic enzymes and preliminary data reveal hundreds of phosphoproteins in developing oilseed rape and soybean. Identification and quantification of phosphoprotein control mechanisms in developing seed of three different oilseeds will provide the basis for understanding the regulatory networks involved in oilseed development and will help in designing new strategies for crop improvement. This proposal aims to unravel networks of phosphoproteins and protein kinases primarily involved in the regulation of metabolic processes during the oil and protein accumulation phase of seed development in three different oilseed plants, oilseed rape, soybean, and Arabidopsis thaliana. In addition to this fundamental scientific objective, the broader impacts of this proposal include: (1) organizing proteomics-themed workshops and developing web-based proteomics tutorials targeting High School teachers and students, respectively; (2) training plant scientists at the undergraduate through post-doctoral levels in the emerging discipline of proteomics; (3) recruiting under-represented students through University-sponsored undergraduate research programs and fellowships; (4) developing techniques for phosphopeptide identification and quantification using the emerging technology of absolute quantification (AQUA) peptides; (5) generating synthetic AQUA peptide tools for the plant protein phosphorylation community; and (6) public dissemination of phosphoproteomics data in multiple, user-intuitive formats to provide new directions for the scientific community to modify seed metabolism for the benefit of health and environment. For website see http://oilseedproteomics.missouri.edu