Time/space Patterns of Microsatellite DNA Variation in Relation to Patch Structure of Southern Ocean Krill, Euphausia Superba

微卫星DNA变异的时空模式与南大洋磷虾斑块结构的关系

• 批准号: 0338195
• 负责人: Ann Bucklin
• 金额: $ 6.61万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0338195&HistoricalAwards=false
• 关键词: Time; space; Patterns; Microsatellite; DNA

The proposed study will use genetic markers to examine population genetic diversity and structure of the Southern Ocean krill, Euphausia superba (Crustacea, Euphausiacea). This effort builds upon previous work by one of the project participants to develop these genetic markers and the appropriate protocols for E. superba. Molecular genetic analysis is planned for archived samples collected during Southern Ocean cruises to the Western Antarctic Peninsula (WAP) during 2001 and 2002. Temporal and spatial patterns of genetic diversity and structure will be described in the context of krill swarms, bathymetry of the collection region, and mesoscale ocean circulation. Specimens of E. superba will be identified from alcohol-preserved zooplankton samples. Krill from different cruises and diverse regions of the sampled domain will be used to screen 20 to 40 genetic markers to determine levels of genetic differences between krill collected in different regions and at different times of the year in the Southern Ocean. The long-term goal of this effort is to contribute to our understanding of the population dynamic processes determining recruitment and maintenance of Southern Ocean krill. Broader impacts for this effort include training of an undergraduate student; international collaboration in research and education with researchers and students in Europe; and new understanding of the genetic architecture of a pivotal species in a polar ecosystem that is likely to be impacted by global change.

本研究将利用遗传标记对南大洋磷虾（Euphausia superba）的种群遗传多样性和结构进行研究。这项工作建立在先前项目参与者之一开发这些遗传标记和适当的超级大肠杆菌方案的工作基础上。计划对2001年和2002年南大洋航行至南极半岛西部（WAP）期间收集的存档样本进行分子遗传分析。遗传多样性和结构的时空格局将在磷虾群、采集区域的水深测量和中尺度海洋环流的背景下进行描述。将从酒精保存的浮游动物样本中鉴定出大叶藻的标本。来自不同邮轮和采样区域不同区域的磷虾将被用来筛选20到40个遗传标记，以确定在南大洋不同地区和不同时间收集的磷虾之间的遗传差异水平。这项工作的长期目标是有助于我们了解决定南大洋磷虾招募和维持的种群动态过程。这项工作的更广泛影响包括培训一名本科生；与欧洲的研究人员和学生在研究和教育方面进行国际合作；对极地生态系统中可能受到全球变化影响的关键物种的遗传结构有了新的认识。