ZooGen, a DNA Sequence Database for Calanoid Copepods and Euphausiids: an OBIS Tool for Uniform Standards of Species Identification

ZooGen，桡足类和磷虾科 DNA 序列数据库：物种鉴定统一标准的 OBIS 工具

• 批准号: 0003884
• 负责人: Ann Bucklin
• 金额: $ 50万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0003884&HistoricalAwards=false
• 关键词: ZooGen; DNA; Sequence; Database; Calanoid

An international partnership is proposed to create a zooplankton genomic (ZooGen) database of DNA type sequences for calanoid copepods and euphausiids. The ZooGen database will be designed to include all species of these groups and to allow expansion to additional zooplankton groups. The ZooGen partnership includes four P.I.s and thirteen expert taxonomic consultants from seven countries. Zooplankton samples will be sorted from existing archival collections, obtained in coordination with planned oceanographic research efforts, and collected during National Marine Fisheries Service field surveys. The taxonomic experts will confirm species' identifications; DNA sequencing will be done at the University of New Hampshire and, in some cases, in other partners' laboratories. For each species, a DNA type sequence will be determined for a portion of the mitochondrial cytochrome oxidase I (mtCOI) gene; multiple mtCOI sequences will be included as necessary to reflect intraspecific variation. The ZooGen database will be designed, created, managed, maintained, and distributed as part of the proposed work; the data will be integrated into the Ocean Biogeographical Information System (OBIS). Education and outreach efforts will include: training of graduate students at UNH and UW; a hands?on Molecular Systematics Workshop at UNH during Summer, 2001; exchanges between the P.Ls, international partners, and their colleagues and students; inclusion of ZooGen molecular and environmental data in the OBIS database, among others; creation and maintenance of a project web site; production of a CD with the ZooGen database and simple search tools; and informal education with the assistance of UNH Sea Grant Educators and Marine Docents.

建议建立一个国际合作伙伴关系，建立浮游动物基因组（ZooGen）数据库的DNA类型序列的哲水蚤桡足类和磷虾。ZooGen数据库的设计将包括这些群体的所有物种，并允许扩展到其他浮游动物群体。ZooGen合作伙伴包括来自七个国家的四名P.I.和十三名专家分类顾问。浮游动物样本将从现有的档案收藏中分类，与计划的海洋学研究工作协调获得，并在国家海洋渔业服务实地调查期间收集。分类专家将确认物种的鉴定; DNA测序将在新罕布什尔州大学进行，在某些情况下，将在其他合作伙伴的实验室进行。对于每个种属，将确定线粒体细胞色素氧化酶I（mtCOI）基因的一部分的DNA类型序列;必要时将包括多个mtCOI序列，以反映种内变异。ZooGen数据库将作为拟议工作的一部分进行设计、创建、管理、维护和分发;数据将被纳入海洋生物地理信息系统（OBIS）。教育和推广工作将包括：在联合国大学和华盛顿大学培训研究生;一个手？2001年夏季在联合国大学举办的分子系统学讲习班;专业人员、国际合作伙伴及其同事和学生之间的交流;除其他外，将ZooGen分子和环境数据纳入OBIS数据库;创建和维护一个项目网站;制作一张带有ZooGen数据库和简单搜索工具的光盘;在联合国海洋赠款教育工作者和海洋讲解员的协助下，