U.S. GLOBEC: Stage-Specific Distribution and Abundance of the Copepods, Pseudocalanus Moultoni and P. Newmani on George's Bank

聚合酶链反应（PCR）扩增的BUCKLIN序列特异性基因将用于鉴定和区分两个类calanoid桡足动物pseudoalanus moultoni和P. newmani的各个阶段的个体。这两个物种的幼虫、幼鱼和成年雌鱼的时间和空间分布将在美国GLOBEC西北大西洋野外研究第二阶段在乔治海岸进行的大规模调查中从浮游动物样本中确定。每月将对分布的空间格局进行分析，以确定具体阶段的分布如何与世界银行的流通格局相互作用。图像分析将提供每个阶段个体的大小估计，以确定物种之间的生长和发育速度是否不同。将与生物物理建模人员合作，将每个物种的阶段结构种群置于现实的流场中。据推测，乔治河岸上的伪蝇的生活史在地理分布和生殖生态方面存在差异。＊＊＊

9632840 BUCKLIN Sequence-specific gene amplification by the polymerase chain reaction (PCR) will be used to identify and discriminate individuals of two calanoid copepod species Pseudocalanus moultoni and P. newmani, at all stages. Temporal and spatial distribution of larval, juvenile, and adult female stages of both species will be determined from zooplankton samples during the broad-scale surveys of U.S. GLOBEC Northwest Atlantic Field Studes Phase 2 on Georges Bank. Spatial patterns of distribution will be characterized monthly to determine how stage-specific distributions interact with circulation patterns on the Bank. Image analysis will provide size estimates for individuals of each stage to ascertain whether rates of growth and development differ between species. Collaborative work with bio-physical modellers will be conducted to place the stage-structured populations of each species in realistic flow fields. It is hypothesized that the life histories of the Pseudocalanus spp. on Georges Bank differ in ways that are suited to the geographic distribution and reproductive ecology of the species. ***