SGER: Novel Devices for Controlling Nanojet Droplet Emulsions (NanoDEs) in Microfluidic Channels

SGER：用于控制微流体通道中纳米喷射液滴乳液 (NanoDE) 的新型装置

• 批准号: 0341793
• 负责人: Abraham Lee
• 金额: $ 6.88万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2005-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0341793&HistoricalAwards=false
• 关键词: SGER; Novel; Devices; Controlling; Nanojet

We propose to design and optimize water-in-oil droplet emulsions using novel microfluidic devices with sub-femtoliter volume control and rapid generation rates (1000 drops/sec). To achieve these goals, we will investigate the nanoscale interfacial forces that control droplet generation, droplet fission, droplet fusion, and intra-droplet mixing/reactions, with and without the presence of amphiphilic molecules. Preliminary results demonstrate microfluidic devices capable of controlling the local shear forces between oil and water interfaces to generate nanojets for droplet emulsions (nanoDEs). These devices generated monodispersed droplet emulsions (0.5m-50m diameter) at high rates (100 drops/sec) corresponding to the input flow rates. Our target is to generate droplet emulsions 100 nm in diameter and droplet generation rates greater than 1,000 drops/sec. In contrast to what has currently been done by others, the proposed research aims to devise new techniques and platforms for forming each individual vesicle with complete nanoscale control of parameters in order to "program" its size, shape, compositional structure, and ultimately its functions and properties.Deliverables for the project include new instrumentation for directed self-assembly of amphiphilic vesicles (AV), new methods to precisely control nanoscale self-assembly process, new characterization methods and tools to monitor vesicle interactions and reactions, the design of new manufacturing processes to implement biological cell machinery in the artificial "transporter vesicles", and understanding of interfacial tension hydrodynamic flow for droplet/vesicle self-assembly.The grant will also make an impact on infrastructure for research and education. The new equipment from this grant and other required existing instruments for the project will be housed in the soon-to-be built building for the California Institute for Telecommunications and Information Technology (Cal-(IT)2).Through this project, the PI intends to introduce a "Freshman Seminars" course to promote nanobiotechnology to incoming freshmen. This will have the broader impact in attracting future new US citizens as well as underrepresented groups to pursue research careers in nano science and engineering.

我们建议设计和优化油包水液滴乳液，使用新型微流体装置与亚飞升体积控制和快速生成速率（1000滴/秒）。为了实现这些目标，我们将研究纳米级的界面力，控制液滴生成，液滴裂变，液滴融合，液滴内混合/反应，有和没有两亲性分子的存在。初步结果表明，微流体装置能够控制油和水界面之间的局部剪切力，以产生用于液滴乳液（nanoDE）的纳米射流。这些装置以对应于输入流速的高速率（100滴/秒）产生单分散液滴乳液（0.5 μ m-50 μ m直径）。我们的目标是生成直径为100 nm的液滴乳液，液滴生成速率大于1，000滴/秒。与目前其他人所做的相比，拟议的研究旨在设计新的技术和平台，以形成每个单独的囊泡，并对参数进行完全的纳米级控制，以便“编程”其大小，形状，组成结构，并最终实现其功能和特性。该项目的可行性包括用于两亲性囊泡（AV）定向自组装的新仪器，精确控制纳米级自组装过程的新方法，监测囊泡相互作用和反应的新表征方法和工具，在人工“转运囊泡”中实现生物细胞机器的新制造过程的设计，以及对液滴/囊泡自组装的界面张力流体动力学流动的理解。该赠款还将对研究和教育的基础设施产生影响。该项目的新设备和其他所需的现有仪器将被安置在加州电信和信息技术研究所（Cal-（IT）2）即将建成的大楼中。通过该项目，PI打算引入“新生研讨会”课程，以向新生推广纳米生物技术。这将在吸引未来的新美国公民以及代表性不足的群体追求纳米科学和工程的研究事业方面产生更广泛的影响。