Intercellular Signaling in Vibrio Harveyi

哈维氏弧菌的细胞间信号转导

• 批准号: 0343821
• 负责人: Bonnie Bassler
• 金额: --
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0343821&HistoricalAwards=false
• 关键词: Intercellular; Signaling; Vibrio; Harveyi

Quorum sensing is a process that allows bacteria to communicate using secreted chemical signaling molecules called autoinducers. This process enables a population of bacteria to collectively regulate gene expression, and therefore behavior. In general, processes controlled by quorum sensing are ones that are unproductive when undertaken by an individual bacterium but become effective when undertaken by the group. For example, quorum sensing controls bioluminescence, secretion of virulence factors, sporulation, and conjugation. We now know that intra- and inter-species cell-cell communication occurs, and this combination of communication abilities was first described, and has been most thoroughly studied, in the model luminous bacterium Vibrio harveyi. The project focuses on V. harveyi, with the aims of exploring the molecular mechanisms used for quorum sensing controlled gene regulation, further defining the members of the quorum sensing regulon, determining the hierarchy of gene expression, and exploring how information from the intra- and inter-species autoinducers is integrated, processed and transduced to control community-wide behavior. Teaching and service related activities are being undertaken as part of this project. These activities include mentoring of undergraduate, graduate, and postdoctoral fellows, instruction in Princeton University undergraduate and graduate courses, Direction of the Princeton Graduate Program in Molecular Biology, and Chairing of the Princeton University Biology Umbrella Development Program, and the Committee for Scientific Direction, Curriculum Development, and Faculty Recruitment. A high school outreach program is being developed for teaching science to local children and teens. Science exhibits are being developed for Science Museum in NYC. Editorial duties will be fulfilled on the boards of Molecular Microbiology, Genetics, Journal of Bacteriology, and Cellular an Molecular Proteomics. Grant and fellowship review work will be performed for NSF, Keck, Damon Runyon, ASM and AAM.

群体感应是一个过程，允许细菌使用分泌的化学信号分子（称为自诱导物）进行交流。这个过程使细菌群体能够共同调节基因表达，从而调节行为。一般来说，由群体感应控制的过程是由单个细菌进行时无生产力，但由群体进行时变得有效的过程。例如，群体感应控制生物发光、毒力因子的分泌、孢子形成和接合。我们现在知道，种内和种间的细胞间通讯发生，这种通讯能力的组合首次被描述，并已被最彻底的研究，在发光细菌哈维氏弧菌模型。该项目的重点是哈维氏弧菌，目的是探索用于群体感应控制基因调控的分子机制，进一步定义群体感应调节子的成员，确定基因表达的层次结构，并探索来自种内和种间自诱导物的信息如何整合，加工和转导以控制整个社区的行为。作为该项目的一部分，正在开展教学和服务相关活动。这些活动包括本科生，研究生和博士后研究员的指导，普林斯顿大学本科和研究生课程的指导，普林斯顿大学分子生物学研究生课程的方向，以及普林斯顿大学生物学伞开发计划的主持，以及科学方向，课程开发和教师招聘委员会。一个高中推广计划正在制定中，用于向当地儿童和青少年教授科学。科学展品正在为纽约的科学博物馆开发。《分子微生物学》、《遗传学》、《细菌学杂志》和《细胞和分子蛋白质组学》的编辑职责将由这些杂志的董事会履行。将为NSF、Keck、Damon Runyon、ASM和AAM进行赠款和奖学金审查工作。