The Complete Vibrio harveyi Genome Sequence

完整的哈维弧菌基因组序列

• 批准号: 0522498
• 负责人: Bonnie Bassler
• 金额: $ 35.78万
• 依托单位: Princeton University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0522498&HistoricalAwards=false
• 关键词: Complete; Vibrio; harveyi; Genome; Sequence

The widespread view of bacteria as primitive asocial organisms has been drastically altered in the past decade by the finding that, using a process called quorum sensing, bacteria communicate using mixtures of secreted chemical signals. Quorum sensing allows bacteria to synchronize gene expression, and therefore behavior, on a population-wide scale, and thus allows them to take on characteristics once assumed to be restricted to higher organisms. Vibrio harveyi, a free-living bioluminescent marine bacterium, has been at the forefront of this paradigm shift, because it was the first bacterium shown to communicate with multiple chemical signals and to be able to communicate across species boundaries. This research will determine the complete V. harveyi genome sequence. The genome sequence will enable an understanding of intra- and inter-species cell-cell communication and how bacteria integrate, process, preserve, and react to multiple sensory inputs. Comparing the V. harveyi genome sequence to other sequenced Vibrio species (Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio fischeri) should enhance understanding of the unique biology, ecology, and disease determinants of this clinically and economically important group of aquatic pathogens. The broader impacts of this work are that quorum sensing studies are providing insight into information processing at the cellular level, the evolution of multi-cellular organisms, and population-level cooperation. The PI teaches undergraduate and graduate courses and she is the Director of Graduate Studies. She sits on many committees and panels, performs editorial duties, runs several science outreach programs, and is an active research mentor.

细菌是原始的非社会生物的普遍观点在过去的十年中已经被发现彻底改变了，细菌使用一种称为群体感应的过程，通过分泌化学信号的混合物进行交流。群体感应允许细菌在整个种群范围内同步基因表达，从而同步行为，从而使它们具有曾经被认为仅限于高等生物的特征。哈维弧菌（Vibrio harveyi）是一种自由生活的生物发光海洋细菌，它一直处于这种范式转变的前沿，因为它是第一个被证明可以通过多种化学信号进行交流的细菌，并且能够跨越物种界限进行交流。本研究将确定哈维氏弧菌的完整基因组序列。基因组序列将有助于了解物种内和物种间的细胞-细胞通讯，以及细菌如何整合、处理、保存和对多种感官输入作出反应。将哈维弧菌基因组序列与其他已测序的弧菌物种（霍乱弧菌、副溶血性弧菌、创伤弧菌和费氏弧菌）进行比较，可以增进对这一具有重要临床和经济意义的水生病原体的独特生物学、生态学和疾病决定因素的了解。这项工作的更广泛的影响是群体感应研究为细胞水平的信息处理、多细胞生物的进化和种群水平的合作提供了见解。PI教授本科和研究生课程，她是研究生学习主任。她是许多委员会和小组的成员，履行编辑职责，运行几个科学推广项目，并且是一个积极的研究导师。