Collaborative Research: Evolution of information processing in the Vibrio fischeri pheromone-signaling network
合作研究:费氏弧菌信息素信号网络中信息处理的演变
基本信息
- 批准号:2029725
- 负责人:
- 金额:$ 18.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-10-01 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Pheromone signaling controls many important bacterial behaviors, ranging from benign symbiotic relationships between bacteria and their hosts to colonization of human tissues and organs by bacterial pathogens. The aim of this project is to understand how the local environment (e.g., the environment in the host) affects pheromone signaling and bacterial behaviors such as colonization. This project combines molecular-based microbiology methods with physical and mathematical tools to develop a more complete experimental and theoretical picture of this process. The project will enhance education by training graduate and undergraduate students to perform cross-disciplinary laboratory research. These research experiences will prepare the students for a wide range of careers in the biosciences. The project also includes a formal training experience for secondary science teachers, primarily from high schools with large numbers of students from under-served populations. The most important elements of Vibrio fischeri pheromone signaling are the LuxI/LuxR signal-synthase/receptor, which produces and detects a 3OC6 homoserine lactone (HSL) pheromone, and the AinS/AinR synthase/receptor system, which employs a C8 HSL pheromone to drive a different signaling cascade. The complexity of these systems is enhanced by the crosstalk generated by LuxR's recognition of both HSL signals, by the fact that both Lux and Ain are modulated in response to the environment, and by the presence of positive feedback in both the Lux and Ain cascades. The primary goal of this project is to understand how the system gathers and integrates information from its environment. The specific aims are to (i) understand how the pivotal regulator LuxR and its variants integrate information from the two HSL signals, (ii) identify the activation states of the broader pheromone-signaling circuitry and explore how environmental inputs lead to specific outputs, and (iii) test the hypothesis that the architecture of this signaling system allows spatially-heterogeneous environments to trigger population-wide responses over extended distance and time scales. The project will use cytometry and gene reporter methods to characterize LuxR interactions and system states. It will also use microfluidics and microscopy to explore signaling dynamics in heterogeneous environments. The results are expected to yield new insights into how bacteria use environmental cues to control population-level behaviors.
信息素信号控制着许多重要的细菌行为,从细菌与宿主之间的良性共生关系到细菌病原体对人体组织和器官的定植。这个项目的目的是了解当地环境(例如,寄主环境)如何影响信息素信号和细菌行为,如定殖。该项目将基于分子的微生物学方法与物理和数学工具相结合,以开发这一过程的更完整的实验和理论图景。该项目将通过培训研究生和本科生进行跨学科的实验室研究来加强教育。这些研究经验将为学生在生物科学领域的广泛职业生涯做好准备。该项目还包括为中学科学教师提供正式培训体验,这些教师主要来自高中,这些学校有大量学生来自服务不足的人群。费氏弧菌最重要的信息素信号转导元件是Luxi/LuxR信号合成酶/受体,它产生和检测3OC6高丝氨酸内酯(HSL)信息素,以及INS/AinR合成酶/受体系统,它利用C8 HSL信息素驱动不同的信号级联反应。这些系统的复杂性由于LuxR对两个HSL信号的识别产生的串扰、Lux和Ain对环境的响应而被调制的事实以及Lux和Ain级联中的正反馈的存在而增加。该项目的主要目标是了解系统如何从其环境中收集和集成信息。其具体目的是(I)了解关键调控因子LuxR及其变体如何整合来自两个HSL信号的信息,(Ii)识别更广泛的信息素信号电路的激活状态,并探索环境输入如何导致特定的输出,以及(Iii)测试该信号系统的体系结构允许空间异质环境在扩展的距离和时间尺度上触发全种群响应的假设。该项目将使用细胞学和基因报告方法来表征LuxR的相互作用和系统状态。它还将使用微流体和显微镜来探索不同环境中的信号动力学。预计这一结果将为细菌如何利用环境线索控制种群水平的行为提供新的见解。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Eric Stabb其他文献
Eric Stabb的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Eric Stabb', 18)}}的其他基金
6th ASM Conference on Cell-Cell Communication in Bacteria; October 16-19, 2017, Athens, Georgia
第六届 ASM 细菌细胞间通讯会议;
- 批准号:
1735551 - 财政年份:2017
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Collaborative Research: Evolution of information processing in the Vibrio fischeri pheromone-signaling network
合作研究:费氏弧菌信息素信号网络中信息处理的演变
- 批准号:
1716232 - 财政年份:2017
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Collaborative Research: Experimental Evolution of Peptidoglycan in the Bacterial Symbiont Vibrio Fischeri
合作研究:细菌共生弧菌费氏弧菌中肽聚糖的实验进化
- 批准号:
1557964 - 财政年份:2016
- 资助金额:
$ 18.29万 - 项目类别:
Continuing Grant
5th ASM Conference on Cell-Cell Communication in Bacteria, October 2014 in San Antonia, Texas
第五届 ASM 细菌细胞间通讯会议,2014 年 10 月在德克萨斯州圣安东尼奥举行
- 批准号:
1440104 - 财政年份:2014
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Sensing more than a quorum: The role of pheromones in the light-organ symbiont Vibrio fischeri
感知不仅仅是群体:信息素在光器官共生体费氏弧菌中的作用
- 批准号:
1121106 - 财政年份:2011
- 资助金额:
$ 18.29万 - 项目类别:
Continuing Grant
Collaborative Research: Iron limitation, carbon metabolism and siderophore production in marine bacteria - a systems biology approach
合作研究:海洋细菌中的铁限制、碳代谢和铁载体产生——一种系统生物学方法
- 批准号:
0929081 - 财政年份:2009
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Collaborative Research: Use of genome enabled tools to understand symbiosis
合作研究:使用基因组工具来理解共生
- 批准号:
0841480 - 财政年份:2009
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
REU Site: Research in Prokaryotic Biology
REU 网站:原核生物学研究
- 批准号:
0755182 - 财政年份:2008
- 资助金额:
$ 18.29万 - 项目类别:
Continuing Grant
CAREER: Symbiotic Role of Bacterial Bioluminescence
职业:细菌生物发光的共生作用
- 批准号:
0347317 - 财政年份:2004
- 资助金额:
$ 18.29万 - 项目类别:
Continuing Grant
相似国自然基金
Research on Quantum Field Theory without a Lagrangian Description
- 批准号:24ZR1403900
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
Cell Research
- 批准号:31224802
- 批准年份:2012
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research
- 批准号:31024804
- 批准年份:2010
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research (细胞研究)
- 批准号:30824808
- 批准年份:2008
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Research on the Rapid Growth Mechanism of KDP Crystal
- 批准号:10774081
- 批准年份:2007
- 资助金额:45.0 万元
- 项目类别:面上项目
相似海外基金
Collaborative Research: Holocene biogeochemical evolution of Earth's largest lake system
合作研究:地球最大湖泊系统的全新世生物地球化学演化
- 批准号:
2336132 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Collaborative Research: LTREB: The importance of resource availability, acquisition, and mobilization to the evolution of life history trade-offs in a variable environment.
合作研究:LTREB:资源可用性、获取和动员对于可变环境中生命史权衡演变的重要性。
- 批准号:
2338394 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Continuing Grant
Collaborative Research: NSF-BSF: Under Pressure: The evolution of guard cell turgor and the rise of the angiosperms
合作研究:NSF-BSF:压力之下:保卫细胞膨压的进化和被子植物的兴起
- 批准号:
2333889 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Collaborative Research: NSF-BSF: Under Pressure: The evolution of guard cell turgor and the rise of the angiosperms
合作研究:NSF-BSF:压力之下:保卫细胞膨压的进化和被子植物的兴起
- 批准号:
2333888 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Continuing Grant
Collaborative Research: LTREB: The importance of resource availability, acquisition, and mobilization to the evolution of life history trade-offs in a variable environment.
合作研究:LTREB:资源可用性、获取和动员对于可变环境中生命史权衡演变的重要性。
- 批准号:
2338395 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Continuing Grant
Collaborative Research: Evolution of acquired phototrophy by organelle sequestration in Mesodinium ciliates
合作研究:中纤毛虫通过细胞器隔离获得的光养进化
- 批准号:
2344640 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Collaborative Research: Holocene biogeochemical evolution of Earth's largest lake system
合作研究:地球最大湖泊系统的全新世生物地球化学演化
- 批准号:
2336131 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Collaborative Research: NSF-BSF: Under Pressure: The evolution of guard cell turgor and the rise of the angiosperms
合作研究:NSF-BSF:压力之下:保卫细胞膨压的进化和被子植物的兴起
- 批准号:
2333890 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant
Collaborative Research: Referential alarm calling as a window into the mechanisms and evolution of a complex cognitive phenotype
合作研究:参考警报呼叫作为了解复杂认知表型的机制和演化的窗口
- 批准号:
2417581 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Continuing Grant
Collaborative Research: Bridging the atomic scale and the mesoscale in the characterization of defect production and evolution in high entropy alloys
合作研究:在高熵合金缺陷产生和演化表征中连接原子尺度和介观尺度
- 批准号:
2425965 - 财政年份:2024
- 资助金额:
$ 18.29万 - 项目类别:
Standard Grant