Calcium Signaling in Plants: Analysis of Vacuolar Calcium Transporters and Regulators

植物中的钙信号传导：液泡钙转运蛋白和调节剂的分析

• 批准号: 0344350
• 负责人: Kendal Hirschi
• 金额: --
• 依托单位: Baylor College of Medicine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0344350&HistoricalAwards=false
• 关键词: Calcium; Signaling; Plants; Analysis; Vacuolar

Regulation of endomembrane calcium (Ca2+) transporters, which control the duration and amplitude of cytosolic Ca2+ spikes, plays a pivotal role in translating a generic cytosolic Ca2+ increase into a specific biological response. In plant biology, the precise contribution made by endomembrane Ca2+ transporters to the intricate process of Ca2+ signaling has not been delineated. The working hypothesis addressed in this project is that vacuolar transporters play a formidable role in shaping cytosolic Ca2+ levels. The PI will use the Arabidopsis vacuolar H+/ Ca2+ exchanger CAX1 to identify molecules that regulate this transporter. Experiments that perturb vacuolar Ca2+ transporters will elucidate the role that vacuolar Ca2+ sequestration plays in adapted responses and assess the spatiotemporal profile of cytoplasmic Ca2+ transients. These experiments will greatly strengthen our fundamental understanding of Ca2+ signaling in plants.

内膜钙转运蛋白调控胞浆钙峰的持续时间和幅度，在将胞浆钙增加转化为特异性生物反应中起着关键作用。 在植物生物学中，内膜Ca 2+转运蛋白对复杂的Ca 2+信号传导过程的精确贡献尚未被描述。 在这个项目中解决的工作假设是，液泡转运蛋白在塑造胞质Ca 2+水平发挥了巨大的作用。PI将使用拟南芥液泡H+/Ca 2+交换器CAX 1来鉴定调节这种转运蛋白的分子。扰动液泡钙转运蛋白的实验将阐明液泡钙螯合在适应性反应中的作用，并评估细胞质钙瞬变的时空分布。这些实验将大大加强我们对植物中Ca 2+信号的基本理解。