Taking a breather: Characterizing the role of H+/Ca transport in anoxia tolerance

喘口气：表征 H /Ca 转运在缺氧耐受中的作用

• 批准号: 2042513
• 负责人: Kendal Hirschi
• 金额: $ 71.91万
• 依托单位: Baylor College of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2042513&HistoricalAwards=false
• 关键词: Taking; breather; Characterizing; role; H+

Plants experience a dynamic environment where numerous stress conditions may be encountered. For example, flooding induces several stresses: limited oxygen availability, changes in temperature, reduced light. Anoxia, the removal of oxygen, is a consequence of waterlogging and submergence of plants. During this stress, plant tissues significantly reduce the rate of energy production, affecting many aspects of metabolism. This project examines how fluctuations in calcium (Ca) within plant cells can impact anoxia tolerance. The investigators have discovered that removal of a specific Ca transporter appears to buffer the plant against anoxic conditions. Here imaging experiments will visually address how this change effects how Ca moves, where it is localized, and how the temporal and spatial changes in Ca can impact anoxia tolerance. To complement these photographic approaches, biochemical and genetic experiments will address the mechanisms of Ca movement. Approaches to measure changes in protein and nucleic acids will be used to further define how removal of a specific Ca transporter can impact the plant’s utilization of energy. One broad impact of this work will be the development of a unique research collaboration with faculty and students from Houston Community College (HCC). HCC is an open-admission, public school that while serving one of the most diverse populations in the US, does not usually provide research experiences. These HCC students will both learn and contribute to this project. The efforts of this work will lead to a better understanding of how plants adapt to their environment. In the future, engineering-modulated Ca movement in plants could circumvent adverse effects of anoxic conditions to mitigate climate change-induced crop loss.Oxygen deficiency (hypoxia) and absence (anoxia) are severe physiological stresses in plants. Most commonly they occur when extreme weather events lead to flooding, which causes widespread crop losses. Membrane transporters may be essential components of anoxia tolerance because they are involved in signal transduction and regulation of metabolic processes. Mutations in the Arabidopsis vacuolar cation/proton exchangers (CAXs) cause striking tolerance to anoxia. This robust phenotype was unexpected: a loss-of-function mutation causing a gain of function. The majority of CAX RNAs are unchanged during hypoxia, highlighting the limits of RNA profiling as an exclusive means of gene discovery. A series of genetic, omics, membrane transport, physiological, cell and elemental imaging experiments will be used to characterize the role of CAXs during anoxia that will demonstrate their role in signaling, cation transport and metabolism. A highly skilled, diverse team will use a multi-disciplinary approach including plant genetics, membrane transport and elemental imaging. As part of the broader impacts, and as a further means of discovery, a Course-based Undergraduate Research Experience (CURE) at Houston Community College (HCC) will be implemented. HCC is an open-admission public school that, while serving one of the most diverse populations in the US, does not typically provide research experiences. In short, characterizing the role of CAXs in anoxia tolerance will lead to a greater understanding of plant signal transduction. Furthermore, this knowledge will eventually aid in the development of flood-resistant crop plants, reducing crop loss during extreme weather events.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物经历了一个动态的环境，在那里可能会遇到许多胁迫条件。例如，洪水会引起几种压力：氧气供应受限、温度变化、光照减少。缺氧，即氧气的去除，是植物受涝和淹没的结果。在这种逆境中，植物组织显著降低能量产生的速率，影响新陈代谢的许多方面。该项目研究植物细胞内钙离子的波动如何影响耐缺氧能力。研究人员发现，移除一种特定的钙转运体似乎可以缓冲植物在缺氧条件下的生长。在这里，成像实验将直观地研究这种变化如何影响钙的移动方式，它被定位在哪里，以及钙的时间和空间变化如何影响缺氧耐受性。为了补充这些摄影方法，生化和遗传实验将解决钙移动的机制。用来测量蛋白质和核酸变化的方法将被用来进一步确定移除特定的钙转运体如何影响植物的能量利用。这项工作的一个广泛影响将是与休斯顿社区学院(HCC)的教职员工和学生发展一种独特的研究合作。哈佛大学是一所开放招生的公立学校，虽然为美国最多元化的人群之一提供服务，但通常不提供研究经验。这些美国大学的学生将在这个项目中学习并做出贡献。这项工作的努力将使人们更好地了解植物如何适应环境。在未来，工程调控的植物钙运动可以规避缺氧条件的不利影响，以减轻气候变化导致的作物损失。缺氧(缺氧)和缺氧(缺氧)是植物中严重的生理胁迫。最常见的情况是，当极端天气事件导致洪水时，洪水会导致大范围的农作物损失。膜转运蛋白可能是耐缺氧的重要组成部分，因为它们参与信号转导和代谢过程的调节。拟南芥空泡阳离子/质子交换器(CAX)的突变导致对缺氧的显著耐受性。这种强健的表型是出乎意料的：一种导致功能获得的功能丧失突变。大多数CAX RNA在缺氧期间没有变化，突显了RNA图谱作为基因发现的唯一手段的局限性。一系列遗传学、组学、膜转运、生理、细胞和元素成像实验将被用来表征CAX在缺氧过程中的作用，从而证明它们在信号转导、阳离子转运和代谢中的作用。一个高技能、多样化的团队将使用包括植物遗传学、膜运输和元素成像在内的多学科方法。作为更广泛影响的一部分，以及作为进一步发现的手段，休斯顿社区学院将实施以课程为基础的本科生研究体验(CURE)。哈佛大学是一所开放招生的公立学校，虽然为美国最多元化的人群之一提供服务，但通常不提供研究经验。简而言之，鉴定CAX在耐缺氧中的作用将有助于更好地理解植物的信号转导。此外，这一知识最终将有助于发展抗洪作物，减少极端天气事件中的作物损失。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses.

液泡 H /Ca 转运蛋白 CAX1 参与淹没和缺氧应激反应。

• DOI: 10.1093/plphys/kiac375
• 发表时间: 2022
• 期刊: Plant physiology
• 影响因子: 7.4
• 作者: Yang,Jian;Mathew,InyElizebeth;Rhein,Hormat;Barker,Richard;Guo,Qi;Brunello,Luca;Loreti,Elena;Barkla,BronwynJ;Gilroy,Simon;Perata,Pierdomenico;Hirschi,KendalD
• 通讯作者: Hirschi,KendalD

Generating Reproducing Anoxia Conditions for Plant Phenotyping

产生用于植物表型分析的繁殖缺氧条件

• DOI: 10.21769/bioprotoc.4603
• 发表时间: 2023
• 期刊: BIO-PROTOCOL
• 影响因子: 0.8
• 作者: Mathew, Iny;Rhein, Hormat;Green, Ardawna;Hirschi, Kendal
• 通讯作者: Hirschi, Kendal