CAREER: Metal Transport by the Primitive Monolobal Transferrin

职业：原始单叶转铁蛋白的金属运输

• 批准号: 0348960
• 负责人: Ann Valentine
• 金额: $ 51.5万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0348960&HistoricalAwards=false
• 关键词: CAREER; Metal; Transport; Primitive; Monolobal

This CAREER award by the Inorganic, Bioinorganic, and Organometallic Chemistry Program supports work by Professor Ann M. Valentine at Yale University to study the metal-carrying properties of the monolobal transferrin protein. In vertebrates and other higher organisms, iron(III) is carried in the serum by transferrin. These transferrins are two-lobed proteins, in which the front and back halves or lobes of each protein molecule are highly similar to one another, and each lobe has a binding site for one metal atom. A precursor monolobal form was recently discovered in a marine invertebrate, the ascidian or sea squirt. The availability of this monolobal protein enables investigations into how the capacity evolved to carry metals in an oxidizing environment, and specifically whether transferrin was once a general metal carrier that was optimized to bind iron most tightly. The objectives are to isolate and characterize the monolobal transferrin from the ascidian Ciona intestinalis, to measure the tightness and establish the mechanism of metal binding, and to determine the mechanisms and conditions for metal release. This project investigates an ancient form of the protein which transports iron in the blood. The research is directed toward understanding the evolution of this protein and how it became more selective for iron. Research will be integrated with educational activities, featuring the development of an introductory course for non-majors that uses bioinorganic chemistry as a platform on which to teach fundamental chemistry, and a program of mentoring and scientific outreach.

这个职业奖由无机，生物无机和有机化学计划支持教授安M。Valentine博士在耶鲁大学研究单叶转铁蛋白的金属携带特性。 在脊椎动物和其他高等生物中，铁（III）通过转铁蛋白在血清中携带。 这些转铁蛋白是双叶蛋白质，其中每个蛋白质分子的前半部分和后半部分或叶彼此高度相似，并且每个叶具有一个金属原子的结合位点。最近在一种海洋无脊椎动物海鞘或海鞘中发现了一种前体单叶状体。 这种单叶蛋白的可用性使得能够研究在氧化环境中携带金属的能力如何演变，特别是转铁蛋白是否曾经是一种被优化以最紧密地结合铁的一般金属载体。 目的是从海鞘中分离和表征单叶转铁蛋白，测量紧密性并建立金属结合机制，并确定金属释放的机制和条件。该项目研究了一种古老的蛋白质形式，它在血液中运输铁。 这项研究旨在了解这种蛋白质的进化以及它如何对铁更具选择性。 研究将与教育活动相结合，为非专业学生开发介绍课程，使用生物无机化学作为基础化学教学平台，以及指导和科学推广计划。