Interactions of Biomolecules and Bacteria with Titanium at the Mineral Microbe Frontier

矿物微生物前沿生物分子和细菌与钛的相互作用

• 批准号: 1412373
• 负责人: Ann Valentine
• 金额: $ 33万
• 依托单位: Temple University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1412373&HistoricalAwards=false
• 关键词: Interactions; Biomolecules; Bacteria; Titanium; Mineral

In this project funded by the Environmental Chemical Sciences Program of the Chemistry Division, Professor Ann Valentine of Temple University is studying how biological molecules and whole microorganisms bind to and dissolve titanium oxide surfaces. Titanium is one of the most abundant elements on earth, but its role in biology is, at best, obscure. Titanium oxides are the most prevalent form of titanium in the environment, but they are normally very inert. Iron-scavenging biomolecules unlock an activity that is otherwise disfavored: the release of large quantities of titanium ions into solution. This research is revealing the role of titanium in biology and, conversely, how biology impacts the environmental chemistry of titanium.This project focuses on the interactions of iron-scavenging siderophores and bacteria with titanium dioxide surfaces, and on the chemical consequences of those interactions. The "siderophore"-mediated mobilization of titanium ions from titanium dioxide is being characterized quantitatively. The oxide surface and materials properties before, after, and during dissolution is being probed by using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy as well as microscopy and related techniques. Microbe/oxide and biomolecule/oxide interactions are being studied by using the bacterium Rhodococcus ruber GIN-1, which adheres avidly to titanium dioxide and incorporates titanium into its biomass. Titanium dioxide surface binding proteins are being identified and characterized, and their abundance and properties correlated to whole-organism phenomena. Titanium uptake is being quantified and intracellular titanium binding molecules isolated. Rhodocuccus ruber GIN-1 siderophores are being isolated and characterized. Research activities are integrated with a program to improve teaching and learning in introductory undergraduate chemistry at Temple University. Environmental bioinorganic chemistry is featured in chemistry outreach efforts. The broader impacts of this work include potential technological benefits from new strategies for anchoring organic molecules to titanium dioxide surfaces, and from the identification of the first native titanium-biomolecule complex and potentially a titanium enzyme.

在这项由化学系环境化学科学计划资助的项目中，坦普尔大学的安·瓦伦丁教授正在研究生物分子和整个微生物如何结合和溶解二氧化钛表面。钛是地球上含量最丰富的元素之一，但它在生物学中的作用充其量也是鲜为人知的。钛氧化物是环境中最常见的钛形式，但它们通常非常惰性。清除铁的生物分子开启了一种原本不受欢迎的活动：将大量钛离子释放到溶液中。这项研究揭示了钛在生物学中的作用，以及反过来，生物如何影响钛的环境化学。本项目重点研究铁清除铁载体和细菌与二氧化钛表面的相互作用，以及这些相互作用的化学后果。“铁载体”介导的钛离子从二氧化钛中的动员正在被定量地表征。利用衰减全反射傅里叶变换红外光谱(ATR-FTIR)以及显微技术和相关技术对氧化物表面和材料在溶解前、溶解后和溶解过程中的性质进行了研究。微生物/氧化物和生物分子/氧化物的相互作用正在使用红色红球菌GIN-1细菌进行研究，这种细菌热衷于附着在二氧化钛上，并将钛结合到其生物量中。二氧化钛表面结合蛋白正在被鉴定和表征，它们的丰度和性质与整个生物体现象相关。正在对钛摄取进行量化，并分离出细胞内的钛结合分子。Rhodocuccus ruber gin-1铁载体正在被分离和鉴定。研究活动与坦普尔大学改善本科生化学入门课程的教与学的计划相结合。环境生物无机化学是化学推广工作的特色。这项工作的更广泛影响包括将有机分子锚定在二氧化钛表面的新策略带来的潜在技术好处，以及第一个天然钛-生物分子复合体和潜在的钛酶的鉴定。