Interactions of Biomolecules and Bacteria with Titanium at the Mineral Microbe Frontier

矿物微生物前沿生物分子和细菌与钛的相互作用

• 批准号: 1708793
• 负责人: Ann Valentine
• 金额: $ 33万
• 依托单位: Temple University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1708793&HistoricalAwards=false
• 关键词: Interactions; Biomolecules; Bacteria; Titanium; Mineral

This award is funded by the Environmental Chemical Sciences Program in the Division of Chemistry. Professor Ann M. Valentine of Temple University is supported to explore the interaction of titanium oxide surfaces with metal-scavenging biomolecules and/or microorganisms. Titanium is very abundant both in the environment and in organisms, and increasingly used in consumer products and industrial applications. Titanium is not widely appreciated as a bioactive element as it has a reputation for extreme inertness and insolubility in water. This research examines the ability of titanium dioxide to stick to (adhere) and release from well-characterized biomolecules and from a bacterium. Iron-scavenging biomolecules bind titanium especially tightly and keep it soluble and stable. They can also cause the release of large quantities of titanium ions into solution. This research sheds light on the impact of microorganisms on the environmental chemistry of titanium and reveals the biological role of titanium. The research is integrated with a new outreach program for rising high school juniors called Philadelphia Environmental Chemistry Explorations (PECE). Environmental bioinorganic chemistry is used as a platform to broaden participation in science. This project focuses on the interactions of iron-scavenging siderophores and bacteria with titanium dioxide (TiO2) surfaces. This inquiry sits at the intersection of inorganic and bioinorganic chemistry, biogeochemistry, and materials and environmental chemistry. Siderophores bind strongly to TiO2, and some siderophores dissolve titanium ions from the metal oxide surface, significantly remodeling the mineral in the process. Electronic spectroscopy, elemental analysis, and mass spectrometry reveal metal ion concentrations and speciation in solution. The oxide surface and properties before, during, and after dissolution are being probed using Attenuated Total Reflectance-Fourier Transfer Infrared (ATR-FTIR) as well as microscopy and related techniques. The mutual interference of titanium and iron is a special focus. Microbe/oxide and biomolecule/oxide interactions are being studied using the bacterium Rhodococcus ruber GIN-1, which adheres to titanium dioxide and incorporates significant quantities of titanium into its biomass. Titanium dioxide surface binding proteins are being identified and characterized. Their abundance and properties are correlate to whole-organism phenomena. Titanium uptake is being quantified and intracellular titanium-binding molecules isolated. A research program is maintained that educates and trains undergraduates and graduate students at the interface of inorganic chemistry and biochemistry. Research activities are integrated with chemistry outreach and with a new program for rising high school juniors. This program uses environmental bioinorganic chemistry as a platform to broaden participation in science.

该奖项由化学部环境化学科学计划资助。教授安M.天普大学的Valentine被支持探索氧化钛表面与金属清除生物分子和/或微生物的相互作用。钛在环境和生物体中都非常丰富，并且越来越多地用于消费品和工业应用。 钛作为生物活性元素并没有得到广泛的认可，因为它在水中具有极强的惰性和不溶性。这项研究考察了二氧化钛粘附（粘附）和从特征良好的生物分子和细菌中释放的能力。清除铁的生物分子与钛结合得特别紧密，使其保持可溶性和稳定性。它们还可以导致大量钛离子释放到溶液中。这项研究揭示了微生物对钛的环境化学的影响，并揭示了钛的生物作用。这项研究与一项名为费城环境化学探索（PECE）的新的外展计划相结合。环境生物无机化学被用作扩大科学参与的平台。该项目的重点是铁清除铁载体和细菌与二氧化钛（TiO 2）表面的相互作用。该研究位于无机和生物无机化学，生物地球化学，材料和环境化学的交叉点。铁载体与TiO 2强烈结合，一些铁载体从金属氧化物表面溶解钛离子，在此过程中显着重塑矿物。电子光谱、元素分析和质谱揭示了溶液中金属离子的浓度和形态。使用衰减全反射-傅立叶变换红外（ATR-FTIR）以及显微镜和相关技术探测溶解前、溶解期间和溶解后的氧化物表面和性质。钛和铁的相互干扰是一个特别的焦点。微生物/氧化物和生物分子/氧化物的相互作用正在研究使用细菌红球菌GIN-1，它坚持二氧化钛，并纳入大量的钛到其生物质。二氧化钛表面结合蛋白正在被鉴定和表征。 它们的丰度和性质与整个生物体现象相关。钛的摄取被量化，细胞内的钛结合分子被分离。一个研究计划是保持教育和培训本科生和研究生在无机化学和生物化学的接口。研究活动与化学外展和一个新的计划，为高中三年级上升相结合。该计划使用环境生物无机化学作为平台，以扩大科学的参与。

项目成果

Photochemistry of ferritin decorated with plasmonic gold nanoparticles

• DOI: 10.1039/c8en01000e
• 发表时间: 2019-01
• 期刊: Environmental Science: Nano
• 作者: Elizabeth B. Cerkez;K. Dutton;Y. Ghidey;Mark A. Kukulka;A. Valentine;D. Strongin

TiO 2 exposure alters transition metal ion quota in Rhodococcus ruber GIN-1

TiO 2 暴露改变红球菌 GIN-1 中的过渡金属离子配额

• DOI: 10.1039/c9mt00305c
• 发表时间: 2020
• 期刊: Metallomics
• 影响因子: 3.4
• 作者: Gallo, Annastassia D.;Zierden, Mark R.;Profitt, Lauren A.;Jones, Kayleigh E.;Bonafide, Christopher P.;Valentine, Ann M.
• 通讯作者: Valentine, Ann M.